The use of non-destructive methods to detect egg hatching properties could increase efficiency in commercial hatcheries by saving space, reducing costs, and ensuring hatching quality. For this purpose, a hyperspectral imaging system was built to detect embryo development and vitality using spectral and morphological information of hatching eggs. A total of 150 green shell eggs were used, and hy...

Although it is well documented that hatching asynchrony in birds can lead to competitive and developmental hierarchies, potentially greatly affecting growth and survival of nestlings, hatching asynchrony may also precipitate modulations in neuroendocrine development or function. Here we examine sibling variation in adrenocortical function in postnatally developing, asynchronously hatching Ameri...

The objective of this study was to evaluate the impact common embryological culturing methods and blastocyst morphology on cell-free DNA (cfDNA) content spent media performance non-invasive preimplantation genetic testing for aneuploidies (niPGT-A). Human embryos were group cultured until day 4 when they washed, transferred into individual fresh droplets (∼20μl) a mature blastocyst. Spent colle...

Abstract:  The optimum dose of UV irradiation to produce gynogenesis rainbow trout, Oncorhynchus mykiss with emphasis of Hertwing effect and photoreactivation (PR) was investigated. For this purpose, the sperm of rainbow trout were inadiated with UV at 2010±200 µw.cm-2 intensity in different alternatives of 0, 1, 3, 5, 8, 10, 15, 20, 25, 35 and 45 minutes and allowed to fertilize normal ova; th...

The number of children born since the origin of Assisted Reproductive Technologies (ART) exceeds 5 million. The majority seem healthy, but a higher frequency of defects has been reported among ART-conceived infants, suggesting an epigenetic cost. We report the first whole-genome DNA methylation datasets from single pig blastocysts showing differences between in vivo and in vitro produced embryo...

culture filtrates (cf) of two species of the nematophagous fungi, arthrobotrys oligospora and arthrobotrys conoidesat three concentrations (25%, 50% and 100%) of stock, were tested on the mortality of second stage juveniles (j2) and egg hatching rate of meloidogyne incognita and meloidogyne javanica. results showed that the percent juvenile mortality was directly proportional to concentration o...