A lot of attention has been paid to elucidate the mechanisms of enzyme inactivation and aggregation so that stabilization strategies can be specifically applied to prevent thermal and/or physical denaturation of enzymes in industrial processes. In the present study, we have investigated the mechanisms of aggregate formation of two model enzymes, a-amylase from Bacillus subtilis and soybean b-am...

A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable alpha-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fe...

Saltpans are an extreme environments, that inhabits organisms are survive in terribly high level of salinities, high temperatures and endure severe solar radiations. The present study was carried out to isolate, screen and optimize amylase enzyme producing bacteria from saltpan environment at Marakkanam. The results of present study was revealed that a total 25 bacterial strains were isolated o...

α-Amylase as an important industrial enzyme has been widely used in starch processing, detergent, and paper industries. To improve expression efficiency of recombinant α-amylase from Bacillus licheniformis (B. licheniformis), the α-amylase gene from B. licheniformis was optimized according to the codon usage of Pichia pastoris (P. pastoris) and expressed in P. pastoris. Totally, the codons enco...

The membrane protein complex translocase mediates the translocation of bacterial proteins. In this complex, the SecY, SecE, and SecG proteins constitute an integral membrane domain. Sequence comparison revealed a potential secG-like gene in the gram-positive soil bacterium Streptomyces lividans. Chromosomal deletion of this gene resulted in a sporulation defect and an overall deficiency in secr...

Background: Corynebacterium glutamicum (C. glutamicum) is a potential host for the secretory production of the heterologous proteins. However, to this date few secretion-type gene expression systems in C. glutamicum have been developed, which limit applications of C. glutamicum in a secretory production of the heterologous proteins.Objectives: In this stu...

Bacillus subtilis subsp. stercoris [ster’co.ris. L. gen. n. stercoris, of compost, from which the strain was isolated]. Grows optimally at 45 C (range 24–50), pH of 7 (range 5–9), and grew in the presence of 7% NaCl. Facultative anaerobe by fermentation, forms white irregular colonies 1–2 mm in diameter when grown on dTSA, cells are straight rods 4–5 lm length by 1 lm width, and stain Gram posi...

The alpha-amylase is used extensively in many different industrial sectors and renowned for modifying starch by rupturing 1-4 glycosidic bands. Depending on the intrinsic properties of microorganism, several alpha-amylases with thermostable halotolerant are expressed. In current study, bacteria were isolated from Ergani Makam Mountain. Identification optimization performed. As a result 16S rRNA...

As an extreme environment, soda lakes harbor various haloalkaliphilic microorganisms. Salda Lake is one of the natural lake (pH?9) in Turkey. Haloalkaliphiles are unique microorganisms their ability to live high alkaline and saline conditions, play important role biodegradation bioremediation hydrocarbons. Hence, aims this study were isolate bacteria from water sample Lake, identify these isola...

conclusions the present study suggests that the enzyme obtained from strain b. subtilis can be used as feed supplement in animal diet also for reduction of phosphorus pollution problem in areas of livestock production. materials and methods the present study was conducted in biotechnology laboratory, motilal nehru national institute of technology, allahabad, uttar pradesh, india. to isolate phy...