Upon base composition analysis, oligonucleotides which are labeled at the 3'-terminus with fluorescein or biotin generate an additional, late eluting peak in the HPLC chromatogram. Investigation of this effect revealed the haptens acted as apurinic sites, and phosphodiesterase cleavage of the phosphate bond between the upstream nucleotide and apurinic site is inhibited. Extension of this work w...

BACKGROUND Determinants of left atrial (LA) reservoir function and its influence on left ventricular (LV) function have not been quantified. METHODS AND RESULTS In an open-pericardium, paced (70 and 90 bpm) pig model of LV regional ischemia (left anterior descending coronary constriction), with high-fidelity LV, LA, and RV pressure recordings, we obtained the LA area with 2D automated border ...

We developed an original base plate to support both the patient's head and a Leksell stereotactic skull frame during frame placement in the supine position. The base plate is made of transparent acrylic board with holes at the posterior posts for injection of local anesthetics and maneuver of fixation screws through them. A stable and comfortable position of the patient's head in a supine posit...

Experimental studies of surface tension and density by the maximum bubble pressure method and dilatometric technique were undertaken and the accumulated data for liquid pure components, binary, ternary and multicomponent alloys were used to create the SURDAT data base for Pb-free soldering materials. The data base enabled, also to compare the experimental results with those obtained by the Butl...

The scroll compressor, using R41 OA that has higher density and works at higher pressure than R22, is required to redesign its geometry such as wrap profile. The design parameters of wrap profile such as base circle radius, wrap height, involute end angle and discharge port have to be well determined to obtain high compressor efficiency. At first, a simulation program has been developed to pred...

This paper studies corporate tax competition if it is costly to learn some of the elements that determine the e¤ective tax burden. Search cost may, but need not, eliminate the tax competition pressure. The outcome depends on the boundaries of tax rate and tax base choices. Search cost can explain the empirically observed tax cuts cum base broadening. Keywords: Costly search, tax competition, co...

This paper considers the design and control of a reactive distillation column in which one reactant is consumed and two products are formed (A B+C). The volatilities are B ¿ A ¿ C , i.e. the reactant is intermediate boiling between the two products. The metathesis of 2-pentene is considered as the demonstrative example. The column has a single feed of the intermediate boiling reactant. The dist...

This is an experimental comparison Of two axi-symmetric, isentropic plug nozzles. In one of these (Model No. 1) the afterbody is cylindricaland a secondary flow is bled into the annular base. The other (Model No. 2) has a parabolic afterbody with no base or bleed. In both cases the expansion is all-external and the design pressure ratio is 14. These models were tested over a range of primary an...

We examine variation in mutation dynamics across a single genome (Zea mays ssp. mays) in relation to regional and flanking base composition using a data set of 10,472 SNPs generated by resequencing 1776 transcribed regions. We report several relationships between flanking base composition and mutation pattern. The A + T content of the two sites immediately flanking the mutation site is correlat...