KEY POINTS Cav1.2 channels maintain activity through interactions with calmodulin (CaM). In this study, activities of the Cav1.2 channel (α1C) and of mutant-derivatives, C-terminal deleted (α1CΔ) and α1CΔ linked with CaM (α1CΔCaM), were compared in the inside-out mode. α1CΔ with CaM, but not without CaM, and α1CΔCaM were active, suggesting that CaM induced channel activity through a dynamic int...

With current and upcoming imaging spectrometers, automated band analysis techniques are needed to enable efficient identification of most informative bands to facilitate optimized processing of spectral data into estimates of biophysical variables. This paper introduces an automated spectral band analysis tool (BAT) based on Gaussian processes regression (GPR) for the spectral analysis of veget...

Two closely related binding modes have previously been proposed for the ATP-competitive benzimidazole class of checkpoint kinase 2 (CHK2) inhibitors; however, neither binding mode is entirely consistent with the reported SAR. Unconstrained rigid docking of benzimidazole ligands into representative CHK2 protein crystal structures reveals an alternative binding mode involving a water-mediated int...

BACKGROUND Overflow metabolism is an undesirable characteristic of aerobic cultures of Saccharomyces cerevisiae during biomass-directed processes. It results from elevated sugar consumption rates that cause a high substrate conversion to ethanol and other bi-products, severely affecting cell physiology, bioprocess performance, and biomass yields. Fed-batch culture, where sucrose consumption rat...

The paper reviews thoroughly a variety of issues related to mode estimation. The potential of self-organizing maps as an approach to mode detection is inquired here. The batch version of the standard SOM and a convex adjustment of it are compared with two kernel-based learning rules, namely, the generative topographic mapping and the kernelbased maximum entropy learning rule. A strategy for mod...

A public database of astrophysical (radio and other) catalogs (CATS), has been created at the Special Astrophysical Observatory (SAO). It allows a user to execute a number of operations in batch or interactive mode, e.g., to obtain a list and parameters of catalogs, to extract objects from one or several catalogs by various selection criteria, to perform cross-identification of different catalo...

Sorption of uranium (VI) from aqueous solution onto powdered corn cob has been carried out using batch and fixed-bed technique. The experimental results in batch technique were fitted well with pseudo second-order kinetics model. In the fixed bed technique, Thomas and Bohart-Adams models were evaluated by linear regression analysis for U(VI) uptake in different flow rates, bed heights and initi...

biopharmaceuticals continues to increase from an estimated $12 billion three years ago to $30 billion today. Of the 122 biopharmaceutical products approved in the United States and Europe, 50 are produced using mammalian cells, 39 by prokaryotic cells, 21 using a yeast system, and a further 12 products by undisclosed systems (1). As an increasing number of potential new drugs emerge from the pr...

Real-world knowledge discovery processes typically consist of complex data pre-processing, machine learning, evaluation, and visualization steps. Hence a data mining platform should allow complex nested operator chains or trees, provide transparent data handling, comfortable parameter handling and optimization, be flexible, extendible and easy-to-use. Depending on the task at hand, a user may w...

Introduction Single-Use Bioreactors are now commonly used for Process Development activities, as seeding bioreactors or to produce Drug Substances. The advantages of this equipment have been well demonstrated over the last years on batch/fed-batch processes. Continuous processes were widely applied in the past to increase the overall productivity of small bioreactors or for sensitive molecule p...