−Animals have typically been used in efficacy tests, but there are a number of dissimilarities between humans and animals. To overcome the problems associated with animal testing, a model which is reproduced in vitro with longterm culture with cell growth with in vivo activity must be developed. We made a gel-type dermal equivalent (DE) that contained dermal papilla cells (DPCs) or dermal sheat...

Cell Culture Bioreactors Basic Types of Bioreactors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1 Stirred Tank (Well Mixed) vs. Tubular Reactor (Plug Flow) . . . . . . . . . . . . . . . . . 3 Segregated Bioreactors (Dead Zone Present) Compartmentalized Bioreactors . . 4 Implication When Growth or Reaction Occurs in the Reactor . . . . . ....

Culture solutions of the submerged freshwater macrophytes Hydrilla verticillata and Vallisneria spiralis, separately incubated at 10 g of fresh weight (FW) per liter for three days, were extracted by solid phase extraction (SPE) followed by liquid liquid extraction (LLE), and the acquired ethyl acetate fractions were analyzed by gas chromatograph-mass spectrometry (GC-MS) to identify potential ...

The aim of this study was to produce microbial oil from Lipomyces starkeyi DSM 70296 grown in hemicellulose hydrolysate (H-H). Glucose and xylose were used for batch, fed-batch, repeated fed-batch, and continuous cultures, and H-H was tested at continuous culture. The highest cell and lipid concentrations of 85.4 and 41.8g/L, respectively, were obtained using repeated fed-batch strategy. Contin...

Utilization of acetate by four strains of Butyrivibrio fibrisolvens was influenced by the composition of their growth medium. Growth-limiting glucose concentrations, low availability of CO2 and the presence of sodium lactate all stimulated acetate uptake by three strains. The type strain, D1, utilized acetate if the concentration of acetate added to the medium was at least 15 mumol ml-1. In bat...