The micropropagation of Stevia rebaudiana using stem node segment explants obtained from 2 years-old shrubs were achieved. Cultures were established on Murashige and Skoog (MS) as the medium supplemented with different concentrations of 6-benzylaminopurine (BA) at concentration, 0.0, 0.1 and 0.5 mg/l individually or in combination with Kinetin (Kin) at concentration, 0.0, 0.1 and 0.5 mg/l. The ...

UNLABELLED Endophytic bacteria are harmless in most plant species; and known to boost the growth and development of the host plants probably by secreting growth hormones. The isolation, identification and screening of endophytic bacteria for the plant growth regulators like cytokinin are needed to get the leads for their applications in agriculture sector. We describe the isolation and identifi...

An efficient and reproducible plant regeneration system was developed from cells or tissues of agronomically important Indian sorghum genotypes including 2 commercial cultivars (NSH27 & K8) of Sorghum bicolor (L.) Moench. Callus induction and plant regeneration were achieved on transverse thin cell layers (tTCL) of roots from aseptically germinated 7-day-old seedlings. Callus response depended ...

Cotton (Gossypium hirsutum L.) is one of the most commercially important fiber crops in the world. Compared with other crops, cotton represents a recalcitrant species for regeneration protocols. The development of efficient and rapid regeneration protocol for elite Indian cotton variety could help improve the quality characteristics and biotic or abiotic stress tolerance. Here we report a novel...

An efficient and reproducible protocol for plantlet regeneration from nodal segments of Olive cv 'Frontio' has been developed. Media and explants browning due to exudation of phenolics from the explants were controlled by fortification of the medium with 100 mg/L ascorbic acid. Best establishment of olive explants was observed on half-strength MS salts fortified with 2.0 mg/L 6-benzylaminopurin...

BACKGROUND Vascular calcification contributes to cardiovascular disease in hemodialysis (HD) patients with diabetes. The randomized controlled trial reported here compared the effects of lanthanum carbonate (LC) and calcium carbonate (CC) on vascular stiffness assessed using brachial-ankle pulse wave velocity (ba-PWV), intima-media thickness (IMT), bone mineral density (BMD), and serum markers ...

A simple and sensitive method was developed to separate the carcinogenic polycyclic aromatic hydrocarbon (PAH), benzo[a]pyrene (BaP), and six of its oxidation metabolites generated by rat hepatic microsomes enriched with cytochrome P450 (CYP) 1A1, by high pressure liquid chromatography (HPLC). The HPLC method, using an acetonitrile/water gradient as mobile phase and UV detection, provided appro...

Turkey is an important center of diversity for many plants species including bulbs, rhizomes, tubers, and other high agricultural horticultural importance. These have a special importance as ornamental plants. However, due to urbanization related factors, them are under threat. One these the endemic Muscari racemosum Mill. The current study aimed develop efficient in vitro commercial bulblet pr...

233 Abstract: A. method is described for plant regeneration from unfertilized ovaries isolated from a diploid male sterile sugar beet (Beta vulgaris L.) breeding line that was developed at the Sugar Institute, Ankara, Turkey. Ovary explants were cultured on Murashige & Skoog (MS) medium containing 2.0 mg/l benzylaminopurine (BAP). Two treatments were tested by incubating all of the explants in ...

Cell culture in shake flask and air-lift bioreactor was carried out to exploit the potential of Arnebia sp. for napthoquinone metabolite production. Cell suspension cultures of Arnebia were established from friable callus in liquid MS medium supplemented with 6-benzylaminopurine (BAP) (10 μM) and indole-3-butyric acid (IBA) (5 μM). Growth kinetic studies were done by using settled cell volume a...