Current European Commission (EC) surveillance regulations require discriminatory testing of all transmissible spongiform encephalopathy (TSE)-positive small ruminant (SR) samples in order to classify them as bovine spongiform encephalopathy (BSE) or non-BSE. This requires a range of tests, including characterization by bioassay in mouse models. Since 2005, naturally occurring BSE has been ident...

Hashimoto’s patients have in some cases both stimulating and blocking antibodies [2, 3]. It stands to reason that if the patient is hypothyroid, then blocking antibody avidity and/ or titer would dominate irrespective of the presence of stimulating antibodies. There is a growing list of publications that show the prevalence of TSHR stimulating autoantibodies in HT patients using bioassays. Wall...

Allergy-preventive activity of flower buds of Lonicera japonica THUNB. was found in the 35% EtOH extract (LJ) using an in vivo assay, The assay system uses monitoring of a decrease in blood flow (BF) in the tail vein of mice subjected to sensitization with hen-egg white lysozyme (HEL). Bioassay-guided fractionation of the 35% EtOH extract led to isolation of chlorogenic acid (1) and three known...

A modification of the mouse fetal liver cell bioassay for erythropoietin (ESF) that allows quantitative detection of ESF in human serum is described. The modification consists of (1) correction for the effect of serum iron on 59Fe incorporation into heme and (2) the use of an "internal standard," i.e., a standard ESF preparation dissolved in the assayed test serum. As a result of this modificat...

The in vitro activities of erythromycin, azithromycin, and clarithromycin against 178 clinical isolates from the lower respiratory tract of patients with chronic obstructive pulmonary disease were determined by an agar dilution method. The plates were incubated in air alone or in 5% carbon dioxide. The MICs measured in air alone were lower for most isolates than those measured in 5% carbon diox...

We have developed a computer code called BIOKMOD that we have applied to analyze several sources of uncertainties in the evaluation of internal exposures using bioassay data: (1) Multiple constant and random intakes in occupational exposures taking into account periods without intake (weekends, holidays, etc.) are evaluated, and they are compared with chronic intakes showing that the chronic ap...

Aedes aegypti L. is the mosquito vector of yellow fever, dengue, zika, and chikungunya viruses. The prevention control such diseases usually rely on use chemicals, that can cause harm to human health environment. Vegetable oils with larvicidal activity are used as an alternative tool this insect. This study aimed evaluate vegetable from Caryocar coriaceum, Mauritia fIexuosa, Carapa guianensis, ...

Amalakyadi churna is an Ayurvedic formulation, which is extensively used in traditional system of Indian medicine and by folk practitioner for treating all types of fever, and other common ailments In this study, an equal concentration of ethanolic extracts of Amalakyadi churna and its ingredients (50μg/ml of artificial sea water) were subjected to a bioscreening study to detect the cytotoxic a...

Aqueous and alcoholic extracts of curcuma amda roxb. rhizomes have been evaluated for its anti tumour activity in Swiss albino mice. Alcoholic extract exhibited more potent anti tumour activity than aqueous extract. In-vitro cytotoxicity studies were confirmed by brine shrimp bioassay method. A significant increase in the life span and a decrease in the cancer cell number and tumour weight were...