Optical imaging is crucial for addressing fundamental problems in all areas of life science. With the use of confocal and two-photon fluorescence microscopy, complex dynamic structures and functions in a plethora of tissue and cell types have been visualized. However, the resolution of 'classical' optical imaging methods is poor due to the diffraction limit and does not allow resolution of the ...

The editors introduce the Biomedical Optics Express feature issue, “Optical Imaging and Spectroscopy,” which was a technical area at the 2010 Optical Society of America (OSA), Biomedical Optics (BIOMED) Topical Meeting held on 11–14 April in Miami, Florida. The feature issue includes 23 contributions from conference attendees. © 2010 Optical Society of America OCIS codes: (000.1200) Announcemen...

Tissue engineering has evolved with multifaceted research being conducted using advanced technologies, and it is progressing toward clinical applications. As tissue engineering technology significantly advances, it proceeds toward increasing sophistication, including nanoscale strategies for material construction and synergetic methods for combining with cells, growth factors, or other macromol...

Quantitative three-dimensional (3D) computed tomography (CT) imaging of living single cells enables orientation-independent morphometric analysis of the intricacies of cellular physiology. Since its invention, x-ray CT has become indispensable in the clinic for diagnostic and prognostic purposes due to its quantitative absorption-based imaging in true 3D that allows objects of interest to be vi...

Due to their anisotropic shape, gold nanorods (GNRs) possess a number of advantages for biosystem use including, enhanced surface area and tunable optical properties within the near-infrared (NIR) region. However, cetyl trimethylammonium bromide-related cytotoxicity, overall poor cellular uptake following surface chemistry modifications, and loss of NIR optical properties due to material intrac...

W HILE it is common knowledge that most images can be greatly compressed, compressive sensing (CS) theory has established that such compression can be done during the data acquisition process and then the uncompressed image can be recovered through a computationally tractable optimization procedure such as L1-norm minimization [1]–[4], or greedy algorithms [5]. In the field of biomedical imagin...

We report on the compact optical design of a high-resolution 3D scanning microscope with adaptive optics capability for refocusing with no moving parts designed for clinical research. The optical aberrations arising from refocusing are compensated for as part of the multiconfiguration optical design process. The lateral scanning is provided by a scanning mirror, and the depth scan is provided b...

The second ‘‘Frontiers of Biomedical Imaging Science’’ conference was held at Vanderbilt University in Nashville, TN from June 2 to 5, 2009. The impetus for the ‘‘Frontiers’’ conferences is that current specialized meetings focus mainly on a single modality and/or disease entity, limiting the opportunities for cross-fertilization across different imaging methods and biomedical research areas. F...