Here, we introduce a method for the fast and accurate analysis of DNA methylation based on bisulfite-treated DNA. The target region is PCR amplified using a T7 RNA polymerase promoter-tagged primer. A subsequent in vitro transcription leads to a transcript which contains guanosine residues only at sites that contained methylated cytosines before bisulfite treatment. In a single tube reaction us...

This report describes the application of bisulfite genomic sequencing to samples consisting of between 50 and 200 intact cells. Bisulfite genomic sequencing, originally described by Frommer et al. (5), allows the determination of methylation status of each cytosine residue in a defined sequence for individual strands of genomic DNA. This technique relies on the ability of bisulfite to specifica...