The biochemical era of blood coagulation research begins toward the end of the 19th century, primarily with the work of Alexander Schmidt1 and Paul Morawitz,2 who hypothesized that the fundamental reactions involved in clotting included the activation of prothrombin to thrombin by thromboplastins and the conversion of fibrinogen to fibrin by thrombin. Throughout the first half of the twentieth ...

Coagulation in crayfish blood is based on the transglutaminase-mediated crosslinking of a specific plasma clotting protein. Here we report the cloning of the subunit of this clotting protein from a crayfish hepatopancreas cDNA library. The ORF encodes a protein of 1,721 amino acids, including a signal peptide of 15 amino acids. Sequence analysis reveals that the clotting protein is homologous t...

'LFulbright Research Scholar, Department of Medicine, Oslo University, 1953-1954. 2 Present Address: Hematology Section, Veterans Administration Hospital, Long Beach, California. There is no standard nomenclature for the newer clotting factors. The following terms are used in this paper. Blood thromboplastin is used in its classic meaning of an activity which arises during clotting and which is...

CONTEXT Research into whether cyclooxygenase-2 (COX-2) inhibitors affect thrombosis has been hampered by the lack of a specific assay. Erythrocytes modulate the effect of aspirin on platelets, which suggests that tests of whole blood clotting may be more sensitive. OBJECTIVES To determine whether reflectance spectroscopy of clotting blood generates useful information about coagulation and whe...