To establish effective and efficient control measures for bovine viral diarrhea (BVD) in Japan, a pilot survey on persistently infected (PI) animals in dairy farms was conducted. A total of 5,949 cattle from 79 farms in 11 prefectures were tested; seven cattle in six farms were identified as PI animals. The proportion of farms with PI animals in Japan was calculated as 7.6% (95% confidence inte...

In the current study, blood samples from 319 cows and heifers were studied. Antibodies against BVDV infections in serum samples and BVDV antigens in leukocytes were present in 203 (148 cows and 55 heifers) and 56 (28 cows and 28 heifers) samples, respectively. Although no significant difference was detected between seropositive ŠBVDV (Ag-/Ab+)1 and uninfected ŠBVDV (Ag-/Ab-)1 cows and heifers a...

Detection of bovine viral diarrhea virus in 143 blood samples by virus isolation in cell culture and flow cytometry was performed. The material included 37 samples later shown to originate from persistently infected cattle. Thirty-three samples were positive by virus isolation, and all 37 samples were positive by the flow cytometric assay.

Structure determination using the single isomorphous replacement (SIR) or single-wavelength anomalous diffraction (SAD) methods with weak derivatives remains very challenging. In a recent structure determination of glycoprotein E2 from bovine viral diarrhea virus, three isomorphous uranium-derivative data sets were merged to obtain partially interpretable initial experimental maps. Small differ...

J. Anim. Sci. Vol. 87, E-Suppl. 2/J. Dairy Sci. Vol. 92, E-Suppl. 1 W24 Effect of timing of Mannheimia haemolytica challenge following short-term exposure to bovine viral diarrhea virus type 1b on serum cytokine concentrations and muscle and fat gene expression changes in growing beef steers. L. Carlos-Valdez*1, L. Burciaga-Robles1, D. L. Step2, R. W. Fulton3, A. W. Confer3, U. DeSilva1, and C....

A dairy herd including approximately 50 milking cows and 40 heifers and calves was investigated. This herd was detected with high prevalence of calves persistently infected (PI) with bovine viral diarrhea virus (BVDV). Nine PI animals including a milking cow and 8 newborn calves were detected in the herd within 4 months. Prevalence of PI animals in this herd was estimated 7.0% which was very hi...

AIM The study was conducted to determine the seroprevalence of bovine viral diarrhea virus (BVDV) and hematological features in crossbred dairy cattle in Chittagong, Bangladesh. MATERIALS AND METHODS The antibody against BVDV in crossbred dairy cattle serum was detected by indirect enzyme-linked immunosorbent assay. The association of different categorical variables in the prevalence of BVDV ...

Bovine viral diarrhea virus (BVDV) isolation and antibody survey were performed using 2,758 fetal bovine sera (FBS) collected from slaughterhouses in New Zealand, Australia and the Dominican Republic, and then sent to Japan to manufacture commercial serum for cell culture use. FBS in the Dominican Republic were pooled for each several individuals, and those collected in other countries were sep...

Bovine viral diarrhea viruses (BVDV) have been isolated alone or in combination with other viral and bacterial pathogens in animals diagnosed with bovine respiratory disease (BRD), a disease causing major economic loss to the feedlot industry. The objective of this experiment was to determine the effects of Mannheimia haemolytica challenge after short-term exposure (72 h) to bovine viral diarrh...

Bovine viral diarrhea virus (BVDV) plays a key role in bovine respiratory disease complex, which can lead to pneumonia, diarrhea and death of calves. Current vaccines are not very effective due, in part, to immunosuppressive traits and failure to induce broad protection. There are diverse BVDV strains and thus, current vaccines contain representative genotype 1 and 2 viruses (BVDV-1 & 2) to bro...