When Brucella suis is grown in tissue cultures of normal guinea pig macrophages, the Brucella multiplies significantly without the induction of osmotically sensitive forms. In immune macrophages in the presence of normal guinea pig serum, there is a reduction in the number of intracellular Brucella and no multiplication is seen over a 72-hr period. After 6 hr of exposure to immune macrophages, ...

The microaerophilic nature of Campylobacter fetus has complicated its recovery from human and animal sources. In this study, modifications of brucella agar and broth were tested for enhancement of growth and aerotolerance of 64 strains of C. fetus, representing each subspecies. Brucella agar supplemented with 0.025% each FeSO4 7H2O, sodium metabisulfite, and sodium pyruvate, supported growth of...

Recently, gram-negative bacteria isolated from a variety of marine mammals have been identified as Brucella species by conventional phenotypic analysis. This study found the 16S rRNA gene from one representative isolate was identical to the homologous sequences of Brucella abortus, B. melitensis, B. canis, and B. suis. IS711-based DNA fingerprinting of 23 isolates from marine mammals showed all...

Canine brucellosis is a zoonotic disease caused by Brucella canis. The establishment of intracellular replicative niches of B. canis is mediated by proteins secreted by the type IV secretion system, which is encoded by the virB operon. The characterization of such genes has been conducted in other species of the genus, but not in B. canis. We report the design of a multiplex PCR test for the de...

BACKGROUND The elderly are predisposed to chronic osteomyelitis because of the immunocompromised nature of aging and increasing number of chronic comorbidities. Chronic osteomyelitis may significantly affect the health of the elderly; however, its impact on long-term mortality remains unclear. We conceived this retrospective nationwide population-based cohort study to address this issue. METH...

introduction brucella endocarditis, although an uncommon complication of brucellosis, is the main cause of the mortality related to this disease. the best therapy is a combination of antibiotic administration with valve replacement. after treatment of the first episode of endocarditis, new infection may be occurred as a relapse or reinfection. case presentation in this report, we described a pa...

Osteomyelitis is an acute or chronic inflammatory process of the bone and its related structures secondary to an infection with pyogenic organisms. Because of the variety in disease presentations and pathophysiology of osteomyelitis, it is very difficult to evaluate in clinical studies. Therefore, animal models have been created for in vivo experimentation. A PubMed and OVID search was performe...

Vertebral osteomyelitis is rare in children. The lumbar spine is the most commonly involved region. Vertebral osteomyelitis occurs more frequently in the vertebral body, and involvement of posterior element is rare. Vertebral osteomyelitis results from hematogenous seeding, spread from contiguous infections, and direct inoculation from spinal surgery. Initial symptoms include low back pain, dif...

AIM To review cases of chronic osteomyelitis managed at a private health institution (Ela Memorial Medical Centre, Ilorin, Nigeria) between March 1995 and February 2005. PATIENTS AND METHODS; Case notes and X-rays of the patients who presented at EMMC with chronic osteomyelitis were reviewed retrospectively. Age, sex, sites of bone involvement and outcome of treatment were recorded. Local surgi...

Chronic osteomyelitis is recognised as a very old disease, but acute osteomyelitis appears to have been understood only in the last 50 or so years. Until the latter part of the nineteenth century, acute osteomyelitis was only rarely recognised, except as a complication of open fractures or local sepsis. According to Wilensky (1927) the term osteomyelitis was probably coined by Nelaton in 1844. ...