Microparticulate carriers are widely employed as drug controlled release system. In this work, the Supercritical Assisted Atomization (SAA) technique was used to produce microspheres of Bovine Serum Albumin (BSA), selected as carrier and gentamicin sulphate (GS) as a model drug. The processability of BSA and GS by SAA was studied using water as solvent and microspheres were successfully produce...

The interaction between Avelox and bovine serum albumin (BSA) was investigated at different temperatures by fluorescence spectroscopy. Results showed that Avelox could quench the intrinsic fluorescence of BSA strongly, and the quenching mechanism was a static quenching process with Förester spectroscopy energy transfer. The electrostatic force played an important role on the conjugation reactio...

three new rimantadine schiff bases (rsbs) were prepared, and then the interaction of rsbs with bovine serum albumin (bsa) was investigated using fluorescence, synchronous fluorescence, uv-vis absorption spectroscopy under physiological conditions. the results showed that the three rsbs effectively quenched the intrinsic fluorescence of bsa via static quenching. binding constant (ka), number of ...

Advance protein based nanobiotechnology are now, known for its advanced approach to synthesize various nanomaterials using various newly developed chemical and green technologies to carry out their applications in various fields of life sciences. Now these days, Bovine serum albumin nanoparticles (BSA NPs) are very popular as non vehicle systems for drug delivery used in number of therapeutic s...

Background and objectives Determination of different grain protein fractions in wheat cultivars is an important task analyzing bread baking quality. In many laboratories, the Bradford assay used to determine concentrations solutions. any (including Bradford), ideal use as a standard purified being assayed. absence such absolute reference, another must be selected relative bovine serum albumin (...

background: progress in the field of biology and biochemistry has led to the discovery of numerous bioactive peptides and proteins in the last few decades. delivery of therapeutic proteins/peptides has received a considerable amount of attention in recent years. methods: in this study, a two-step desolvation method was used to produce biodegradable hydrophilic gelatin nanoparticles (gnp) as a d...

Fluid overload is a common complication in peritoneal dialysis (PD) patients. The prognostic importance of enrollment fluid status in long-term PD patients remains to be investigated. The objective of the present study was to investigate the prognostic importance of enrollment fluid status in the long-term survival of PD patients. We enrolled 53 PD patients (mean age: 53 years) from November 20...

Rosmarinic acid (RA) is an importantly and naturally occurring polyphenol from plants of the mint family with potent biological activities. Here, the in vitro interaction of RA with bovine serum albumin (BSA) has been investigated using various biophysical approaches as well as molecular modeling methods, to ascertain its binding mechanism and conformational changes. The fluorescence results de...

Progesterone conjugated to bovine serum albumin (BSA) was used as a probe to study sex differences and the effects of hormonal status on binding of progesterone to crude synaptosomal membrane preparations (P2) derived from the mediobasal hypothalamic-anterior hypothalamic-preoptic area or the corpus striatum. Binding of 125I-labeled BSA linked to progesterone at the 11 position of the steroid (...

Can creatinine clearance in children clear the indexing GFR with BSA from the charge of errors? We read with interest the article of Delanaye and colleagues stating that indexing glomerular filtration rate (GFR) with body surface area (BSA) required mathematical prerequisites to be satisfied, even though many studies reported they were not [1]. Although not the gold standard to measure GFR, cre...