Among the abiotic stresses, drought is a serious limiting factor that reduces rice production and yield stability in rainfed ecosystems. Conventional breeding for drought resistance is slow in attaining progress due to poor understanding of genetic control of drought resistance. Molecular markers help in identification of quantitative trait loci (QTLs) associated with drought resistance traits ...

The cucumber (Cucumis sativus L.) exhibits extensive variations in fruit size and shape. Fruit length is an important agronomic and domesticated trait controlled by quantitative trait loci (QTLs). Nonetheless, the underlying molecular and genetic mechanisms that determine cucumber fruit length remain unclear. QTL-seq is an efficient strategy for QTL identification that takes advantage of bulked...

Complex traits such as crop performance and human diseases are controlled by multiple genetic loci, many of which have small effects and often go undetected by traditional quantitative trait locus (QTL) mapping. Recently, bulked segregant analysis with large F2 pools and genome-level markers (named extreme-QTL or X-QTL mapping) has been used to identify many QTL. To estimate parameters impactin...

ABSTRACT Crown rust of barley, caused by Puccinia coronata var. hordei, occurs sporadically and sometimes may cause yield and quality reductions in the Great Plains region of the United States and Canada. The incompletely dominant resistance allele Rpc1 confers resistance to P. coronata in barley. Two generations, F(2) and F(2:3), developed from a cross between the resistant line Hor2596 (CIho ...

PURPOSE To establish the zebrafish platinum mutant as a model for studying vision defects caused by syndromic albinism diseases such as Chediak-Higashi syndrome, Griscelli syndrome, and Hermansky-Pudlak syndrome (HPS). METHODS Bulked segregant analysis and candidate gene sequencing revealed that the zebrafish platinum mutation is a single-nucleotide insertion in the vps11 (vacuolar protein so...

Eucalyptus gunnii leaves can appear as green or glaucous phenotypes with the latter more desirable for floriculture. The epicuticular wax from these two types were compared morphologically using environmental scanning electron microscopy, chemically using gas chromatography, and molecular markers were found to distinguish the two types using RAPDs and bulked segregant analysis. Both phenotypes ...

Molecular markers are modern diagnostic tools, which may help breeders to solve practical problems. They facilitate cultivar identification, the determination of genetic similarities among breeding stocks and enable the calculation of polymorphism level, heterozygosity or self-pollination rate. But the main expectation with respect to molecular markers is their potential use in marker-assisted ...

Abstract Background Freezing injury, which is an important abiotic stress in horticultural crops, influences the growth and development production area of kiwifruit ( Actinidia Lind1). Among species, arguta has excellent cold resistance, but knowledge relevant to molecular mechanisms still limited. Understanding mechanism underlying resistance for breeding resistance. Results In our study, a po...