CaCl2 treatment followed by heat shock is the most common method for artificial transformation. Here, the cells were transformed using CaCl2 treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. The BL21 strain of Escherichia coli (E. coli) was being susceptible using CaCl2 treatment. Some Cells were ...

Lecithin and lecithin/cholesterol dispersions as well as lysolecithin micelles were used to provide basic information on the mechanism of the interaction of zwitterionic phospholipids with dextran sulfate. The addition of dextran sulfate to lecithin dispersions or lysolecithin micelles in the presence of Ca2+ produced insoluble complexes. At each Ca2+ concentration, the amount of insoluble comp...

Phosphorus solubilizing microorganisms are capable of transforming insoluble phosphorus (P) forms to soluble and bioavailable P. The aim of the present study was to isolate P solubilizing bacteria from forest soils around the rock phosphate (RP) mine of Koh-e-SepidLar in Kohgiluyeh and Boyer Ahmad province and evaluate their ability in Sperber culture medium and wheat straw medium in the presen...

‘Golden Delicious’ apples (Malus domestica Borkh) were pressure-infiltrated at harvest with a 4% CaCl2 solution either without prior heat treatment or following 4 days at 38C. Examination of the apple surfaces from both treatments by low-temperature scanning electron microscopy revealed that heat treatment changed the pattern of epicuticular wax. The epicuticular wax of nonheated fruit exhibite...

We examined the effectiveness of intermittent warming (IW), hot water (HW), salicylic acid (SA), and calcium chloride (CaCl2) on the postharvest life of pomegranate fruit cv. ‘Shishe-Kab’ and extending fruit shelf life during cold storage (3°C). Fruit were subjected to cycles of 1 d at 17 ± 1°C every 6 d of storage at 3°C under IW conditions. Pre-storage treatments were HW (50°C) for 3 min, SA ...