By analyzing DNA fingerprints of chickens from seven well-defined genetic groups, a calibration curve was established relating the degree of inbreeding with the average band frequency, allelic frequency and band sharing. The probe used was bacteriophage M13 DNA and digestion of the genomic DNA was carried out with the MspI restriction enzyme. The analysis also provided an estimate of the averag...

A comparison was made between plasma concentrations of prednisolone measured by both competitive protein binding radioassay (CPB) and radioimmunoassay (RIA) and, with each assay, using a calibration curve generated from individual subject data and from pooling the individual calibration curva data. The plasma samples were obtained from six normal adult male volunteers who were pretreated with d...

We report an on-line coupling of gel permeation chromatography (GPC) to Fourier transform mass spectrometry (FTMS) using a modified commercial electrospray ionization (ESI) interface. Selected oligomer profiles for the sodiated (1+ through 5+ charge states) oligomer ions of a narrow-molecular-weight poly(methyl methacrylate) were generated and used for obtaining a calibration curve. Using the M...

lead is quantitatively retained on 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (5-br-padap)-ammonium tetraphenylborate with microcrystalline naphthalene or by a column method in the ph range 4.0-6.0 from a large volume of aqueous solutions of various samples with preconcentration factor of 60. after filtration, the solid mass consisting of the lead complex and naphthalene was dissolved with 5...

We optimized fluorogenic thrombin-generation assays with regard to sample volume, calibration, analytic corrections, and activation reagents. Lower sample volumes (40 vs 80 microL) were associated with better recovery of thrombin activity, lower interference due to absorbance of light, and higher total thrombin generation (area under the curve), even using internal standards to calibrate plasma...

A method to measure the deuterium concentration in basic solutions, using Attenuated Total Reflection Fourier Transformed Infrared Spectroscopy (ATR-FTIR) is described. Deuterium quantification is based on the O-D bond stretching peak. A calibration curve is drawn, representing the peak intensity versus the deuterium percentage in a neutral solution. No calibration curve can be obtained with ba...

This paper describes a Built-In-Self-Test (BIST) circuit that tests the relative timing jitter of NRZ data and recovered clock of PLL-based CDR. Using the jitter information, the bit error rate and peak to peak jitter can be estimated. This BIST circuit doesn’t need a high resolution and high accuracy delay line to achieve high accuracy measurement. It depends on calibration and curve fitting a...

In structure light measurement systems or 3D printing systems, the errors caused by optical distortion of a digital projector always affect the precision performance and cannot be ignored. Existing methods to calibrate the projection distortion rely on calibration plate and photogrammetry, so the calibration performance is largely affected by the quality of the plate and the imaging system. Thi...

A novel time-of-flight mass calibration method has been developed. In contrast to conventional methods, where the relationship between ion flight time and mass is an arbitrary polynomial equation, this method is based on the physics of ion motion. Parameters needed to describe the physics are numerically optimized using a simplex algorithm. Once these parameters are established, unknown masses ...

In-house cocoa flour RMs instead of CRMs were prepared and checked for its homogeneity and stability. Due to CRMs limited availability in terms of matrices of concern and high production cost when making in-house cocoa flour, RMs are alternatively used for daily quality control in the laboratory. The analysis method for reliable checking of homogeneity and stability of cadmium content in the in...