Background Agrobacterium mediated gene transfer technology offers the potential to introduce novel, high-value traits into selected, elite tree genotypes. Significant progress has been made in the regeneration and genetic transformation of Eucalyptus trees, but transformation efficiency has generally been low for eucalypts [1], especially for elite clonal genotypes. As a preliminary step toward...

The improvement of garlic plants (Allium sativum L.) via biotechnological approaches is currently limited by the lack of an applicable direct gene transfer system. In this paper, we present the development of a genetic transformation system using particle bombardment for gene delivery and immature clove-derived callus as the gene target. Plasmid DNA (pBI221.23), containing the selectable "hpt" ...

Enhanced production of total saponins was obtained from 40 day old suspension cultures of Bacopa monnieri. The callus yielded not only a 166% increased concentration of saponins but also produced two novel prominent bands of saponins compared to the natural plant system. We report here for the first time an increased potential of cell lines of B. monnieri in suspension for production of saponin...

Unselected and sodium sulfate tolerant callus cultures of Brassica napus L. cv Westar were grown on media supplemented with mannitol, NaCl, or Na(2)SO(4). In all cases, growth of tolerant callus, measured on a fresh weight or dry weight basis, was greater than that of unselected callus, which was also subject to necrosis on high levels of salt. Tissue water potential became more negative in bot...

This study investigated the effects of magnetite nanoparticles (MNPs) various sizes (10.77, 20.5, and 29.3 nm), different concentrations (0.5, 1, 2 ppm) on growth production active constituents in Ginkgo biloba L callus. The best medium for callus proliferation was MS supplemented with 1 ppm naphthalene acetic acid (NAA), Kinetin, 0.5 MNPs a size 10 nm, which gave maximum fresh weight percentag...

Plant natural products including alkaloids, polyphenols, terpenoids and flavonoids have been reported to exert anticancer activity by targeting various metabolic pathways. The biological pathways regulated plant can serve as novel drug targets. compounds or their derivatives used for cancer treatment some plant-based which are in clinical trials were discussed. Callus suspension culture with se...