Fracture healing involves the coordinated actions of multiple cytokines. Bone morphogenetic protein 9 (BMP9) is an important factor in bone formation. The present study aimed to investigate the osteogenic potential of bone marrow stem cells (BMSCs) in response to adenoviral (Ad)BMP9, and the early fracture repair properties of AdBMP9 in surgically‑created fractures in osteoporotic rats. Alkalin...

Plant cells have a remarkable ability to induce pluripotent cell masses and regenerate whole plant organs under the appropriate culture conditions. Although the in vitro regeneration system is widely applied to manipulate agronomic traits, an understanding of the molecular mechanisms underlying callus formation is starting to emerge. Here, we performed genome-wide transcriptome profiling of wil...

An efficient plant regeneration system for Pinellia ternata (Thunb) Breit, an important wild Chinese herb, was established. The investigated factors influencing callus induction and plant regeneration included sterilizing agents, explant type, culture medium, and combinations of plant growth regulators in the medium. Ten min treatment of explants with 0.1% HgCl2 was more suitable for sterilizat...

INTRODUCTION: The time required for bridging callus formation following femoral fracture increases with age in the rat (1). While young, 6-week-old rats form bridging callus by 4 weeks after fracture, adult, 26-week-old rats require 10 weeks, and older, 52-week-old rats need in excess of 26 weeks (1). Despite this increased time to healing with age, there was no increase in the time of expressi...

plant tissue culture techniques were used to study the effect of nacl on tropane alkaloids accumulation and proline content of both datura metel and datura stramonium. callus cultures were established for both datura species. however, ms medium supplemented with 1mg/l of both ba and naa was the best for callus production in d. metel, while ms supplemented with 3 mg/l kin and 1 mg/l 2, 4-d was t...

The cactus Mammillaria gracillis Pfeiff. was propagated in vitro on Murashige and Skoog (MS) medium without any growth regulators. At the bases of some plants abundant callus masses developed. Lower agar and higher MS salt concentrations stimulated callus pro­ duction. In the culture, morphologically normal and hyperhydric shoots were regenerated. The growth of crown-gall tumour was induced on ...

Callus formation is a critical step for successful fracture healing. Little is known about the molecular composition and mineral structure of the newly formed tissue in the callus. The aim was to evaluate the feasibility of small angle x-ray scattering (SAXS) to assess mineral structure of callus and cortical bone and if it could provide complementary information with the compositional analyses...

Since bone marrow has been shown to contain osteoprogenitor cells, an experiment was devised to test its effects when injected percutaneously into osteotomies sites in rabbit radii. In this experimental study, the osteogenicity and it's effect on early bone repair of bone marrow grafts were investigated. The purpose of this study was to determine whether bone marrow grafted percutaneously led t...

Chlorogenic acid, a pharmacologically important compound, is a phenolic compound that occurs in certain commonly used medicinal herbs. We looked for the presence of this compound in the callus cultures of Varthemia persica DC (var. persica). We have evaluated the conditions for establishment of callus cultures of V. persica and the in vitro production of chlorogenic acid. Callus was initiated b...