Hydrogenosomes are found in organisms that lack typical mitochondria. Cardiolipin is a phospholipid located exclusively in bacterial membranes and the inner membrane of mitochondria. Here we show, by cell fractionation, thin-layer chromatography, high-pressure liquid chromatography, and matrix-assisted laser desorption ionization-time of flight mass spectrometry that hydrogenosomes of Tritricho...

For many years it has been known that leprosy patients are prone to give positive reactions in the serologic tests for syphil is (STS) in common use. Badger (1) reviewed the literature on the subject up to 193 1. Since that time, several other studies have been made (2-5, 10, 12, 13, 15). almost all of them showing that reactions occur frequenty in STS's in the sera of patients with leprosy. so...

Anionic lipids play a variety of key roles in membrane function, including functional and structural effects on respiratory complexes. However, little is known about the molecular basis of these lipid-protein interactions. In this study, NarGHI, an anaerobic respiratory complex of Escherichia coli, has been used to investigate the relations in between membrane-bound proteins with phospholipids....

Physicians and serologists have faced a number of questions associated with sero-diagnostic reactions in syphilis which have been difficult to answer. For example, it may be asked how is it possible for serum reactions employing lipid antigens, apparently unrelated to the Treponema pallidum, to behave as though they were specifically associated with syphilis, as shown by the remarkable parallel...

Potential of mean force (PMF) calculations are used to characterize the free energy landscape of protein-lipid and protein-protein association within membranes. Coarse-grained simulations allow binding free energies to be determined with reasonable statistical error. This accuracy relies on defining a good collective variable to describe the binding and unbinding transitions, and upon criteria ...

The effect of cardiolipin content on the shape and size of giant palmitoyloleylphosphatidylcholine/cardiolipin vesicles was studied. Unilamellar vesicles were prepared in sugar solution by the method of electroformation, from mixtures containing up to 50% weight ratio of cardiolipin. At room temperature the vesicles containing cardiolipin exhibited abrupt changes in the curvature of the vesicle...

The nature and quantity of the phospholipids of Salmonella typhimurium and Escherichia coli K-12 have been examined. The main classes of phospholipids, phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin have been completely characterized. Four minor compounds have been detected: phosphatidylserine, phosphatidic acid, and two partially characterized lipids. The phospholipid composit...

Since the original report of the isolation of cardiolipin (I), the usefulness of this substance in serodiagnostic tests for syphilis has been demonstrated (2-5). In this laboratory an antigen consisting of a mixture of cardiolipin, lecithin, and cholesterol (4) is now in routine use in complement fixation tests for evidence of syphilis (6). It was therefore important to devise more efficient an...

The lipids of Caulobacter crescentus, a procaryotic species which differentiates into stalked and swarmer cell types, were analyzed. Major lipid classes were purified by chromatography and identified by both chromatographic and chemical methods. Approximately half of the total lipid fraction of this organism consisted of glycolipis, which were primarily monoglucosyldiglyceride and an acylated g...

VP1, a putative alpha-helical antimicrobial peptide (alpha-AMP) inhibited growth of Bacillus subtilis and Escherichia coli at 500microM. The peptide induced stable surface pressure changes in monolayers formed from B. subtilis native lipid extract (circa 4.5mNm(-1)) but transient pressure changes in corresponding E. coli monolayers (circa 1.0mNm(-1)), which led to monolayer disintegration. Synt...