Precise genome editing using engineered nucleases can significantly facilitate biological studies and disease treatment. In particular, clustered regularly interspaced short palindromic repeats (CRISPR) with CRISPR-associated (Cas) proteins are a potentially powerful tool for modifying a genome by targeted cleavage of DNA sequences complementary to designed guide strand RNAs. Although CRISPR/Ca...

Methanobrevibacter oralis, an anaerobic methanogenic archaeon, has been previously isolated from the human oral cavity. Here, sequencing a stool isolate (strain JMR01) yielded a 2.065-Mb genome with a 27.78% G+C content containing a total of 2,042 open reading frames and 3 clusters of regularly interspaced short palindromic repeat (CRISPR) loci with associated Cas proteins.

UNLABELLED Carbonic anhydrases (CAs) are metalloenzymes catalyzing the reversible hydration of carbon dioxide to bicarbonate (hydrogen carbonate) and protons. CAs have been identified in archaea, bacteria and eukaryotes and can be classified into five groups (α, β, γ, δ, ζ) that are unrelated in sequence and structure. The fungal β-class has only recently attracted attention. In the present stu...

1 Système à représenter 3 1.1 Description générale . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 1.2 Les processus associés à la station graphique . . . . . . . . . . . . . . . . . . . . 4 1.2.1 Processus associé au cas d’utilisation “Cas 1” : . . . . . . . . . . . . . . 4 1.2.2 Processus associé au cas d’utilisation “Cas 2” : . . . . . . . . . . . . . . 4 1.3 Les processus ...

Cognitive Architectures (CAs) are the core of artificial cognitive systems. A CA is supposed to specify the human brain at a level of abstraction suitable for explaining how it achieves the functions of the mind. Over the years a number of distinct CAs have been proposed by different authors and their limitations and potentials were investigated. These CAs are usually classified as symbolic and...

Carbonic anhydrases (CAs) are metalloenzymes that are omnipresent in nature. CAs catalyze the basic reaction of the reversible hydration of CO₂ to HCO₃- and H⁺ in all living organisms. Photosynthetic organisms contain six evolutionarily different classes of CAs, which are namely: α-CAs, β-CAs, γ-CAs, δ-CAs, ζ-CAs, and θ-CAs. Many of the photosynthetic organisms contain multiple isoforms of each...

Les sacro-iliites infectieuses méritent d'être mieux connues. Leur diagnostic est souvent retardé en raison d'une symptomatologie trompeuse et des diffcultés d'exploration de l'articulation sacro-iliaque. Notre travail est basé sur une étude rétrospective portant sur les cas de SII, recueillis sur une période comprise entre 1997 et 2008 dans notre centre universitaire Sfax-Tunisie. Le diagnosti...

Amlinger, L. 2017. The type I-E CRISPR-Cas system. Biology and applications of an adaptive immune system in bacteria. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 1466. 61 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-554-9787-3. CRISPR-Cas systems are adaptive immune systems in bacteria and archaea, consisting of a clustered reg...