1 Department of Chemistry and Biochemistry, Massey University, Palmerston North, New Zealand * New Zealand Dairy Research Institute, Palmerston North, New Zealand An endopeptidase has been purified from Lactococcus lactis subsp. crernoris SK11. The enzyme is a 70 kDa monomer, strongly inhibited by the metalloproteinase inhibitors 1,l O-phenanthroline and phosphoramidon but relatively insensitiv...

The fatty liver that develops in insulin-treated, depancreatized dogs and in dogs subjected to ligation of their pancreatic ducts has been the subject of considerable study (1). Soon after its discovery it was shown to be prevented by the feeding of raw pancreas (2), while later findings revealed that it could also be prevented by the feeding of pancreatic juice and certain fractions derived fr...

BACKGROUND Mammalian semen contains a family of closely related proteins known as Binder of SPerm (BSP proteins) that are added to sperm at ejaculation. BSP proteins extract lipids from the sperm membrane thereby extensively modifying its composition. These changes can ultimately be detrimental to sperm storage. We have demonstrated that bovine BSP proteins interact with major milk proteins and...

Dietary proteins are recognized by the gastrointestinal tract to display physiological functions, however, the sensory mechanism of the intestinal mucosa is not known. We examined binding properties between the rat small intestinal brush-border membrane (BBM) and proteins by using a surface plasmon resonance biosensor. BBM and solubilized BBM prepared from the rat jejunum bound to casein immobi...

milk is an important component of a balanced diet and contains many valuable constituents. a considerable part of the milk healthbenefits is related to its proteins, not only for their nutritive value but also for their biological properties. scientific evidence suggests that anticarcinogenic activities, antihypertensive properties, immune system modulation, and other metabolic features of milk...

allergen-specific immunotherapy (ait) has been recently considered as an alternative approach to ameliorate the symptoms of allergen exposure and improvement the patients’ quality of life. dendritic cells (dc) in the forms of tolerogenic or th1-induced cells have been investigated in several studies as one of the promising approaches of ait in allergic diseases. the aim of this study was to eva...

The mammary gland is a system in which the components of the blood stream which are used by body tissues for protein synthesis can be readily investigated (Barry, 1956). Previous work has shown that the mammary gland takes the free essential amino acids from the blood stream to provide the essential amino acids of casein (Barry, 1952; Campbell & Work, 1952; Askonas, Campbell, Godin & Work, 1955...

PURPOSE Despite most epidemiologic studies reporting that an increase in milk intake affects the growth of prostate cancer, the results of experimental studies are not consistent. In this study, we investigated the proliferation of prostate cancer cells treated with casein, the main protein in milk. MATERIALS AND METHODS Prostate cancer cells (LNCaP and PC3), lung cancer cells (A459), stomach...

the purpose of this study is to produce an oil-in-water nano-emulsion by ultrasonication. casein combined with inulin used as continuous phase, while dispersed phase consisted of fish oil. the size of the nano-emulsion and the ph of nano-emulsion were characterized. ultrasound has been used for preparing emulsion by 24 khz intensity for 120 seconds. prepared nano-emulsion was investigated by pa...

BACKGROUND Cow's milk allergy diagnosis many times requires double-blind placebo-controlled food challenge (DBPCFC), which presents high accuracy but involves risks, specifically in infants and anaphylactic patients. The identification of the cut-off values for specific IgE to milk or its components would contribute to cow's milk allergy (CMA) diagnosis. The aim of this study was to compare dis...