The multicopper oxidase CueO had previously been demonstrated to exhibit phenoloxidase activity and was implicated in intrinsic copper resistance in Escherichia coli. Catecholates can potentially reduce Cu(II) to the prooxidant Cu(I). In this report we provide evidence that CueO protects E. coli cells by oxidizing enterobactin, the catechol iron siderophore of E. coli, in the presence of copper...

Parkinson's disease (PD) is the second most common neurodegenerative disorder after Alzheimer's disease. It is characterized by bradykinesia, hypokinesia/ akinesia, rigidity, tremor, and postural instability, caused by dopaminergic (DA) striatal denervation. The prevalence of PD increases from 50 years of age with steep rise after age 60 years. Current treatment of PD relies heavily on replacin...

Here, we provide the NMR spectra and AFM data for antioxidant micelles prepared from amphiphilic PAM-PDA block copolymers composed of a poly(N-acryloyl morpholine) and a redox-active catechol-bearing block with different catechol content. We also provide details of the electrochemical analysis that showed micelles higher catechol content had a similar redox potential with the small catechol com...

When a hot aqueous extract of black tea cools down, a colloidal complex (tea cream) is formed, which poses technological problems in industrial processes e.g. manufacture of instant tea and tea cordials. Removal of 'tea cream' by centrifugation leaves the residual liquor stripped to a great extent of the desirable characteristics of tea. The studies on the nature of tea cream, the mechanics of ...

This study evaluated the effects of inhibitors on polyphenol oxidase (PPO) activity, the effect of the PPO inhibitor tropolone on noodle darkening, and the correlation of PPO activity with darkening of alkaline noodles. The PPO inhibitors tropolone and salicylhydroxamic acid (each at 1 microM) reduced kernel PPO activity by approximately 50% in three hexaploid wheat cultivars but did not inhibi...

As a part of the study to explore the possible strategy for enhancing the shelf life of mango fruits, we investigated the changes in biochemical parameters and activities of ripening associated enzymes of Ashwina hybrid mangoes at 4-day regular intervals during storage at -10°C, 4°C, and 30 ± 1°C. Titratable acidity, vitamin C, starch content, and reducing sugar were higher at unripe state and ...

Yeast and bovine cytochrome c oxidases (COX) are composed of 12 and 13 different polypeptides, respectively. In both cases, the three subunits constituting the catalytic core are encoded by mitochondrial DNA. The other subunits are all products of nuclear genes that are translated on cytoplasmic ribosomes and imported through different transport routes into mitochondria. Biogenesis of the funct...

By definition, a dormant vegetative bud is viable but it fails to grow when subjected to environmental conditions normally favorable for growth. An understanding of the biochemistry of dormant and active buds is needed to serve as a basis for solving the problem of artificially breaking or inducing bud dormancy. The present study was conducted with the aim of adding to existing knowledge concer...

(1) The theme of the survey is that metalloenzymes represent rather unique' metal complexes when compared to the simpler system generally studied by coordination chemists. This theme is introduced by a brief presentation of the historical development of the metalloenzyme field, particularly the contributions of 0. Warburg and D. Keilin. (2) Various types of metal ion—protein interactions and th...