The adrenal glands from 15 guinea pigs aged 90 days were used in the study. Paraffin slices were stained with Mayer haematoxylin and eosin with Masson method and silvered. The histochemical reactions were carried out for catecholamines and the biochemical determinations of catecholamines. Ultrastructural observations were also performed. In the adrenal medulla of sexually mature guinea pigs num...

The stereospecificity of the beta-adrenergic receptor of turkey erythrocyte ghosts was studied. Binding of the specific beta-adrenergic antagonist, propranolol, was found to occur exclusively with the l-stereoisomer. The stereospecificity of catecholamine binding was determined by assaying the ability of the catecholamines to displace [(3)H]propranolol. Binding of the catecholamines was also fo...

The majority of the published reference range data on catecholamines excretion by healthy children is incomplete and often contradictory (1). We assayed in the urines of 127 healthy children the values of the catecholamines (norepinephrine, epinephrine, dopamine) and their methylated metabolites (normetanephrine, metanephrine, 3-methoxytyramine) for the determination of paediatric reference ran...

I analyzed for free and sulfate-conjugated catecholamines and DOPA (3,4-dihydroxyphenylalanine) in four aliquots of a single plasma pool with use of Upjohn's "Cat-A-Kit" (catecholamines radioenzymatic assay kit [3H]. One aliquot was a clear plasma control; the others were supplemented with different concentrations of lysed human erythrocytes, to investigate possible interference by hemolysis wi...

Background The exercise-induced rise in arterial potassium concentration ([K']a) may contribute to exercise hyperpnea and could play a role in exertional fatigue. This study was designed to determine whether the exercise-induced rise in [K']a is altered in patients with chronic heart failure (CHF) and whether physical training affects K' homeostasis. Methods and Results We evaluated 10 subjects...

SYNOPSIS Two patients with phaeochromocytoma having atypical biochemical features are described. Total catecholamine excretion was normal in one and only slightly raised in the other; both had a diagnostic rise in output of metadrenaline and 4-hydroxy-3-methoxymandelic acid whilst 4-hydroxy-3-methoxyphenylglycol excretion was increased in one of them. During hypertensive attacks adrenaline excr...

I describe a relatively simple and rapid procedure for quantitative analysis for unconjugated urinary norepinephrine, epinephrine, and dopamine. Catecholamines are isolated from urine on a carboxylic acid ion-exchange resin (Bio-Rex 70) and eluted with 0.8 mol/L boric acid. The eluates are assayed for the individual catecholamines by "high-performance" liquid chromatography with an octadecylsil...

Thirteen young adults with bronchial asthma and a like number of controls were subjected for 45 minutes to a temperature of 2 degrees C after leaving a temperature of 24 degrees C to which they subsequently returned. Pre-exposure examination included VC, FEV1, MMEF, FEV1/VC%, and urinary catecholamines. The pulmonary measurements were repeated at 15, 80, and 200 minutes after exposure. Catechol...

1. Splenic arterial flow and splenic weight were recorded in cats anaesthetized with sodium pentobarbitone. The responses of the spleen to catecholamines, angiotensin and vasopressin were investigated.2. Catecholamines caused responses mediated by alpha- and beta-adrenoceptors in the arteriolar smooth muscle, but only insignificant beta-adrenoceptor responses could be elicited from the capsular...

We describe an improved method of sample preparation for liquid chromatographic determination of plasma catecholamines. The catecholamines are extracted from plasma by using small, cheaply-made columns of alumina, with or without prior clean-up on commercially available ion-exchange columns. Advantages of this technique over the conventional batch-extraction method of using alumina are speed, c...