PURPOSE The matrix metalloproteinase 3 (MMP3), also known as stromelysin-I, is a key-player for carcinogenesis and tumor growth. A 5A/6A promoter polymorphism is associated with differences in MMP3 activity and has been linked to cancer susceptibility in some studies. In the present study we evaluated the role of this polymorphism for breast cancer risk. EXPERIMENTAL DESIGN A case-control stu...

Objective: To study the associations of stromelysin-1 (MMP3) gene 5A/6A polymorphism with plasma high-sensitivity C-reactive protein (hs-CRP) level in coronary artery disease (CAD) and myocardial infarction (MI). Methods: The MMP3 5A/6A genotypes and plasma hs-CRP levels were determined in 405 non-CAD subjects and 395 angiography-documented CAD patients, 157 with MI and 238 with non-MI. Results...

Cytotox ic T-cel ls were derived from the per i toneal cavity of a C 5 7 B L / 6 mouse immunized with B A L B / c sarcoma Meth A and from the sp leens of B A L B / c x C 5 7 B L / 6 F1 (hereaf ter cal led CB6F1) mice immunized with B A L B / c leukemia R L ~ I . The cel ls were cu l tured in inter leukin 2 and c loned by limiting di lut ion, and their speci f ic i ty was determined by d i rect ...

Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects. In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally re...

The molecular co-chaperone CDC37 is over-expressed in hepatocellular carcinoma (HCC) cells, where it functions with HSP90 to regulate the activity of protein kinases in multiple oncogenic signaling pathways that contribute towards hepatocarcinogenesis. Disruption of these signaling pathways via inhibition of HSP90/CDC37 interaction is therefore a rational therapeutic approach. We evaluated the ...

Idiopathic hypereosinophilic syndrome (HES) and chronic eosinophilic leukemia (CEL) comprise a spectrum of indolent to aggressive diseases characterized by unexplained, persistent hypereosinophilia. These disorders have eluded a unique molecular explanation, and therapy has primarily been oriented toward palliation of symptoms related to organ involvement. Recent reports indicate that HES and C...

Aims. We present an analysis of physical conditions in planetary nebulae (PNe) in terms of collisionally-excited line (CEL) and optical-recombination line (ORL) profiles. We aim to investigate whether line profiles could be used to study the long-standing CEL/ORL abundance-discrepancy problem in nebular astrophysics. Methods. Using 1D photoionization models and their assumed velocity fields, we...

BACKGROUND Single nucleotide polymorphism (SNP) markers are the method of choice for genetic analyses including diversity and quantitative trait loci (QTL) studies. Marker validation is essential for QTL studies, but the cost and workload are considerable when large numbers of markers need to be verified. Marker systems with low development costs would be most suitable for this task. RESULTS ...

Selection of HTC cells in drugs that inhibit ornithine decarboxylase (ODC) has produced two cell lines, HMOA and DH23A/b, that contain increased amounts of more stable ODC. In addition to alterations in ODC, these cells appear to produce modified eukaryotic initiation factor 5A (eIF-5A) at different rates, a reaction that both requires spermidine and is essential for proliferation. Alterations ...

In in tes t ina l mucosa of fed adult rats, cytochrome P-450 content and ac t i v i t y of benzpyrene hydroxyl ase and p-n i t roaniso le O-demethylase are highest in the upper small in test ine and progressively decrease toward the terminal ileum. Among the mucosal cell populations, mature v i l l ous t i p cel ls contain 6 to I0 times more cytochrome P-450 and drug-metabolizing ac t i v i t y...