The role of various cell wall components of nonencapsulated Staphylococcus aureus in phagocytosis and killing was studied. Adsorption of human serum with S. aureus cell walls, or trypsin-digested cell walls, reduced the rate of phagocytosis and killing of S. aureus by human-blood leukocytes when compared to unadsorbed serum. When the various components of the cell walls were used for adsorption...

Apoptosis depends on multiple pathways to execute changes in the biochemistry and structure of the dying cell. Apoptotic DNA degradation is one such pathway that fragments the chromosomes, allowing them to be cleared from the debris of the dead cell. In C. elegans, apoptotic DNA degradation is also important to the highly reproducible pattern of cell killing that occurs during normal developmen...

We have examined the effect of killing of host monocytes infected with bacillus Calmette-Guérin (BCG) on the viability of the intracellular mycobacteria. Peripheral blood monocytes were infected in vitro with a single bacillus per cell and maintained in culture for 6-8 d to allow the bacilli to replicate. Replicating viable BCG were found singly in perinuclear vacuoles bounded by tightly appose...

a radio assay employing 14c-uridine to measure the intracellular killing of candida albicans by phagocytes is presented. the principle of this new method is that after killing of candida albicans by phagocytes rna synthesis stops and therefore uridine uptake is diminished whilst viable candida in cultures would take up uridine and as a result, high cpm counts can be obtained within 135 min. of ...

An in vitro colony formation assay was modified to determine the effects of in vivo 1,3-bis(2-chloroethyl)-1-nitrosourea therapy on tumor cell kill and subsequent clonogenic cell kinetics. The measured surviving fraction must be multiplied by the relative total number of tumor cells for each posttreatment interval in order to eliminate inaccuracies caused by dead cell removal in vivo and the ly...