The phenolic constituent of nonvascular cell walls of wheat (Triticum aestivum L.) coleoptiles, which yields vanillin upon nitrobenzene oxidation, is not lignin as I previously claimed. It seems to be mainly ferulic acid bonded to carbohydrate, probably by an ester linkage. The acid is associated with a fraction of the wall rich in arabinose and xylose, although it is not known whether it is es...

Primary cell walls of Zea mays prepared in a glycerol medium are capable of autolysis in vitro. Autolysis results in solubilization of about 10% of the wall substance during an 8 hour incubation period. Approximately 10% of the solubilized material is glucose and the remainder consists of an unidentified polymer which yields only glucose upon hydrolysis. Cell wall autolysis is a linear function...

[This corrects the article on p. 134 in vol. 43.].

We introduce a general theoretical framework to study the shape dynamics of actively growing and remodeling surfaces. Using this framework we develop a physical model for growing bacterial cell walls and study the interplay of cell shape with the dynamics of growth and constriction. The model allows us to derive constraints on cell wall mechanical energy based on the observed dynamics of cell s...

Addition of cell walls to the peptidoglycan synthetase-acceptor system containing vancomycin (50 mug/ml) prevented the inhibition by the antibiotic. In addition, the inhibition of incorporation of [(14)C]muramyl-pentapeptide into peptidoglycan in the presence of vancomycin was reversed by the addition of cell walls to the assay mixture at 60 min. Cell walls previously saturated with vancomycin ...

Bacteriophage N1 does not irreversibly adsorb to cell walls isolated from its host Micrococcus lysodeikticus strain 1 (ML-1). ML-1 walls do bind the virus in a specific but completely reversibly union. Electron microscopic examination of OsO(4)-treated mixtures of phage and walls revealed phage bound to wall fragments by their tail tips, suggesting that reversible phage attachment to walls invo...

The composition of guard cell walls in sugar beet leaves (Beta vulgaris L.) was studied by using histochemical staining and immunocytochemical detection of cell wall antigens. The findings were compared with those in the walls of epidermal and mesophyll cells. Probing of leaf sections with monoclonal antibodies against pectins, terminal fucosyl residues linked alpha-(1-->2) to galactose, beta-(...

In this paper, the position of crossing linkage between cell wall polysaccharides was studied by determining the digestibility of pectin-depleted walls, the contents of hydrocinamic acids and their dimmers in each fraction of elongated and non-elongated cells. The results showed that Ox. sol. fr. was composed mainly of pectin polysaccharides while 4K sol. fr. was composed both of pectin and hem...

Cell walls made by Bacillus subtilis bacteria grown in D(2)O medium have buoyant densities in CsCl which are different from walls made by cells grown in H(2)O medium. Cell wall turnover was studied by measuring the change in wall buoyant density after a B. subtilis culture was shifted from growth in D(2)O medium to aeration in H(2)O medium. Walls from the hemispherical caps were isolated after ...

Given the social importance of intracranial aneurysm as a major cause of a lethal subarachnoid hemorrhage, clarification of mechanisms underlying the pathogenesis of this disease is essential for improving poor prognosis once after rupture. Previous histopathological analyses of human aneurysm walls have revealed the presence of T cells in lesions suggesting involvement of this type of cell in ...