Objective: Investigations were carried out to isolate bacteria from saline-alkali soils and determined optimized alkaline protease activity and stability produced by a wild strain of bacillus sp. in submerged fermentation (SMF). Methods: Optimum temperature for enzyme activity in the crude extract was 40 ◦C at a pH between 8.0 and 9.0. The studies on pH stability showed that the enzyme...

background and objectives: psychrotrophic bacteria can produce enzymes at low temperatures; this provides a wide biotechnological potential, and offers numerous economical advantages over the use of mesophilic bacteria. in this study, extracellular protease production by psychrotrophic rheinheimera sp. (km459533) was optimized by the response surface methodology. materials and methods: the cult...

graft copolymers of acrylamide on carboxymethyl cellulose (d.s 0.4-0.5) were prepared by the use of ceric ion, ceric ion/reductant molecule initiator systems in aqueous medium. the graft copolymers were characterized by ir spectroscopy. the extent of graft copolymerisation was measured in terms of grafted chains as a function of both ceric ion and ceric ion/reductant molecule concentrations. it...

Objective(s): The purpose of this study was to compare novel sandwich-structured nanofibrous membranes, and coaxial and usual methods, to provide sustained-release delivery of morphine for drug delivery. In this work, synthesis ofnanofibrous cellulose acetate (NFC) was carried out by electrospinning. Methods: A weighed amount of cellulose acetate (CA) pow...

It has already been reported that muscle necrosis induced by various phenylenediamine derivatives are correlated with their autoxidation rate. Now in a more detailed investigation of the cytotoxic mechanism using a model system of isolated hepatocytes and ring-methylated structural isomer durenediamine (DD) we have shown that under aerobic conditions, phenylenediamine induced cytotoxicity and R...

introduction: proteolytic enzymes, specially collagenase, are used to digest extracellular matrix, cells isolation and primary culture. it is important to find new sources of plant or animal protease instead of bacterial or tissue collagenase. in the present research, actinidin, a plentiful protease in kiwifriut, was used to isolate human umbilical vein endothelial cells. materials and methods:...

it has already been reported that muscle necrosis induced by various phenylenediamine derivatives are correlated with their autoxidation rate. now in a more detailed investigation of the cytotoxic mechanism using a model system of isolated hepatocytes and ring-methylated structural isomer durenediamine (dd) we have shown that under aerobic conditions, phenylenediamine induced cytotoxicity and r...