Covalent binding of xenobiotic electrophiles to nucleophilic endogenous biomolecules, e.g. peptides or DNA, is a common molecular initiating event, leading to potentially irreversible toxic effects such as enhanced acute toxicity, skin sensitisation, or mutagenicity. This knowledge provides the basis for the in silico prediction of these toxicities. The potential for a chemical to be reactive c...

BACKGROUND Epigenetic modifications, particularly DNA methylation and posttranslational histone modifications regulate heritable changes in transcription without changes in the DNA sequence. Despite a number of studies showing clear links between environmental factors and DNA methylation, little is known about the effect of environmental factors on the recently identified histone lysine methyla...

In this investigation, Nutlin-3 (Nut3), a novel antitumor drug with low water solubility (<0.1mg/L at 25°C), was loaded into liposomes (Lipo-Nut3), polymeric nanoparticles (NPs-Nut3) and nanoparticles engineered with an antibody direct against Syndecan-1/CD 138 (Syn-NPs-Nut3) to obtain carriers targeted to PEL (primary effusion lymphoma). The physicochemical properties of these carriers were de...

Biogenic amines are compounds commonly present in living organisms in which they are responsible for many essential functions. They can be naturally present in many foods such as fruits and vegetables, meat, fish, chocolate and milk, but they can also be produced in high amounts by microorganisms through the activity of amino acid decarboxylases. Excessive consumption of these amines can be of ...

Fluorescence Correlation Spectroscopy (FCS) was first introduced by Elson, Madge and Webb (1-5) for studying the binding process between ethydium bromide and DNA. When the ethydium dye binds to DNA its fluorescence quantum yield changes by a large factor. It is essentially not fluorescent when free in solution and it becomes strongly fluorescent when bound to double strand DNA. Although the pro...

In the early 90’s, donkeys were in danger of extinction as species. In Italy, there were counted few heads of many breeds but, unfortunately, others had already been extinct. With the donkey in danger, there was a risk of disappearance of a part of ecosystem, a biodiversity particularly significant as it represents humans’ history. All countries around the Mediterranean sea, countries of southe...

many studies on cold environments have been developed over the past two decades. highmountain freshwater presents high variability of nutrients and chemico-physical parameters, showing variations of ph, oxygen concentration, metals and temperature throughout the year. national park of sierra nevada (granada, spain) (37°032 n 03°182 w), has almost 40 lakes that are reported to be both endemic an...

Autocatalytic reaction fronts between two reacting species in the absence of fluid flow, propagate as solitary waves. The coupling between autocatalytic reaction front and forced simple hydrodynamic flows leads to stationary fronts whose velocity and shape depend on the underlying flow field. We address the issue of the chemico-hydrodynamic coupling between forced advection in porous media and ...

The study investigated the basic geomechanical and mineralogical evolution of the bentonite barrier under various experimental boundary conditions which replicated the near-field Thermo-Hydro-Chemico (THC) conditions in a repository. The relationships between the physicochemical alterations and changes in the geotechnical properties have seldom been studied, especially on a consistent dataset. ...