A selective eco-friendly micellar HPLC method was developed for investigation of moxifloxacin and related compounds in the presence its degradation products. Central composite design used to optimize experimental conditions. The proposed is based on isocratic elution a C18 column using 92.5% (v/v) biodegradable aqueous mobile phase containing 0.01 M sodium dihydrogen phosphate, 0.15 dodecyl sul...

A reversed-phase column liquid chromatographic method was developed for the assay of amoxicillin and its preparations. The linear calibration range was 0.2 to 2.0 mg/ml (r = 0.9998), and recoveries were generally greater than 99%. The high-performance liquid chromatographic assay results were compared with those obtained from a microbiological assay of bulk drug substance and capsule, injection...

High efficiency and less run time are the basic requirements of high-speed chromatographic separations. To fulfill these requirements, a new separation technique, ultra-performance liquid chromatography (UPLC), has shown promising developments. A rapid, specific, sensitive, and precise reverse-phase UPLC method is developed for the determination of nabumetone in tablet dosage form. In this work...

An environmentally benign RP-HPLC approach for rapid analysis of glibenclamide in pure form, developed nanoemulsion and commercial tablets was developed and validated in present investigation. The green chromatographic identification was performed on Lichrosphere 250 X 4.0 mm RP C8 column having a 5µm packing as a stationary phase using a combination of ethanol: methanol (50:50 % v/v) as a mobi...

An environmentally benign RP-HPLC approach for rapid analysis of glibenclamide in pure form, developed nanoemulsion and commercial tablets was developed and validated in present investigation. The green chromatographic identification was performed on Lichrosphere 250 X 4.0 mm RP C8 column having a 5µm packing as a stationary phase using a combination of ethanol: methanol (50:50 % v/v) as a mobi...

The stability indicating properties of the USP method for the assay of fentanyl in fentanyl citrate injection were evaluated [1] by analyzing fentanyl drug substance and product after acid, hydrogen peroxide, heat, and light treatment. N-phenyl-N-(4-piperidinyl)propionamide (PPA), which is a known degradation product/process impurity of fentanyl, was not adequately resolved from the fentanyl pe...

Caffeine (CAF) levels in instant regular coffees, instant decaffeinated coffees, and instant teas are determined and compared using spectrophotometric and gas chromatographic methods of determination. Modification of a previously reported spectrophotometric assay for CAF in biological fluids is necessary to reduce interference from theophylline (THP) and theobromine (THB). Readjustment of the b...