The effects of commercial formulations Viburnum opulus and Equisetum arvense extract on anti-cytotoxicity anti-genotoxicity were evaluated at the root tip Allium cepa L. Distilled water (negative control), dimethyl sulfoxide (DMSO), ethyl methane sulfonate (EMS, positive 5% 10% doses V. E. extracts applied for 24 48 hours. Cytotoxicity genotoxicity by calculating %mitotic index %chromosomal abe...

RADIOPROTECTIVE EFFECTS OF GINSENG EXTRACT IN GAMMARAYS INDUCED CHROMOSOMAL DAMAGES OF HUMAN LYMPHOCYTE. Ginsan, a polysaccharide extracted from Panax ginseng and subsequently referred as ginseng, posses various biological properties as an anticancer and antioxidant agent. Ginseng also approved effective against radiation effects through its immunomodulating actions in whole body irradiated mou...

PURPOSE To examine the influence of α-particle radiation exposure from internally deposited plutonium on chromosome aberration frequencies in peripheral blood lymphocytes of workers from the Sellafield nuclear facility, UK. MATERIALS AND METHODS Chromosome aberration data from historical single colour fluorescence in situ hybridization (sFISH) and Giemsa banding (G-banding) analyses, together...

The thymidine analogue, 5'-bromodeoxyuridine (BrdU), is a known mutagen that is routinely introduced into culture media for subsequent Harlequin stain analysis and determination of cell cycle status. Previously, we examined the induction of chromosome aberrations in human peripheral blood lymphocytes (PBL) known to be in their 1st cell division following exposure to a low dose (0.5 Gy, average ...

purpose: to study the distribution of ocular higher-order aberrations (hoas) and mesopic pupil size in individuals screened for refractive surgery. methods: ocular hoas and mesopic pupil size were studied in 2458 eyes of 1240 patients with myopia, simple myopic astigmatism and compound myopic astigmatism and 215 eyes of 110 patients with hyperopia, simple hyperopic astigmatism and compound hype...

Aneuploidy has been well-documented in blastocyst embryos, but prior studies have been limited in scale and/or lack mechanistic data. We previously reported preclinical validation of microarray 24-chromosome preimplantation genetic screening in a 24-h protocol. The method diagnoses chromosome copy number, structural chromosome aberrations, parental source of aneuploidy and distinguishes certain...

Background: Induction of radioadaptive responses in cells pretreated with a low dose radiation before exposure to a high dose is well documented by many investigators. The aim of this study is to determine the frequency of chromosomal aberration in peripheral blood lymphocytes of patients treated by radioiodine (131I) for hyperthyroidism, with or without previous thyroid scan with 99mTc. Materi...

Background Microalgae accumulate lipids when exposed to stressful conditions such as nutrient limitation that can be used to generate biofuels. Nitrogen limitation or deprivation is a strategy widely employed to elicit this response. However, this strategy is associated with a reduction in the microalgal growth, leading to overall poor lipid productivities. Here, we investigated the combined ef...

OBJECTIVE To study the acute toxicity of C25P polypeptide, a CCR5 antagonist, in mice and its carcinogenic effect in vitro. METHODS The acute toxicity of C25P polypeptide in mice was assessed by determining the maximum tolerated dose (MTD). The mice were given C25P at the dose of 3.64 g/kg by tail vein injection, and the control mice received saline (40 ml/kg) injection. The mice were continu...

We describe a stromal predominant Wilms tumor with focal anaplasia and a complex, tumor specific chromosome 11 aberration: a homozygous deletion of the entire WT1 gene within a heterozygous 11p13 deletion and an additional region of uniparental disomy (UPD) limited to 11p15.5-p15.2 including the IGF2 gene. The tumor carried a heterozygous p.T41A mutation in CTNNB1. Cells established from the tu...