PURPOSE To increase the database of genes expressed in human cornea and to gain insights into the molecular basis of keratoconus (KC). METHODS A cDNA library was constructed from KC corneas harvested at keratoplasty and used for expressed sequence tag (EST) analysis. Data were analyzed using grouping and identification of sequence tags (GRIST). Expression of selected clones was examined by RT...

PURPOSE To report two cases of femtosecond laser-assisted small incision deep lamellar endothelial keratoplasty (DLEK) for patients with corneal endothelial decompensation by Fuchs dystrophy and glaucoma METHODS Femtosecond laser (IntraLase; IntraLase Corp., Irvine, CA) with 15 kHz of repetition rate, was used for a 9.5 mm diameter by 400 micrometer thickness donor corneal lamellar dissection...

The following corneal conditions will be considered: (1) pressure oedema; (2) oedema following endothelial damage; (3) persistent folds in Descemet's membrane; (4) vascularization; (5) lipoid infiltration; (6) band-shape degeneration. Oedema of the cornea caused by a sudden elevation of intra-ocular pressure is a diagnostic feature of acute angle-closure glaucoma. If the intra-ocular pressure r...

Aim: To assess graft survival and endothelial cell loss after DSAEK using a pull-through insertion technique with incision width of 3.2mm or 4.1mm. Methods: The medical records of 100 consecutive eyes that had undergone DSAEK at the Turin University Ophthalmology Institute between November 2007 and April 2010 were reviewed. Surgery was performed by a single surgeon and included: descemethorexis...

OBJECTIVES To determine the reported incidence of acute endophthalmitis following cataract extraction over time and to explore possible contributing factors, such as type of cataract incision. METHODS A systematic review of English-language articles was conducted by performing a broad search of PubMed from 1963 through March 2003 using such terms as cataract extraction, endophthalmitis, and p...

A scleral contact lens lies on the sclera, not cornea, which differs from a rigid gas permeable (RGP) lens, and has shown successful clinical outcomes in patients unable to tolerate glasses or RGP lenses because of severely deformed cornea. In addition, forms tear film between cornea that stabilizes ocular surface could be useful therapeutic option. The mini-scleral is option for visual improve...

pus escaped, and subsequently light water dressing was applied. Next morning the bandage was found quite saturated with discharge, and on removing the dressing and applying gentle pressure over the riizht iliac fossa, about half an ounce of clear, serous fluid (peritoneal) oozed out ; on the left side the edges of the incision showed a tendency to heal, without any further purulent accumulation...