teinase inhibitors was included in the lysing solution. To preclude the precipitation of non-specific material, lysates were pre-cleared twice with goat anti-( mouse IgG)agarose, followed by incubation with NB-1, an irrelevant antibody [4]. Digestion of immunoprecipitated antigen for 4 h with trypsin at a final concentration of 1 mg/ml resulted in bands at 21 kDa, 16.5 kDa, 11.5 kDa, 10 kDa, 7....

ObjectivesWe described the prevalence of clofazimine (CFZ) resistance in a multidrug-resistant tuberculosis (MDR-TB) cohort China. We also aimed to identify dynamic changes CFZ susceptibility and its molecular mechanism after exposure bedaquiline (BDQ) and/or CFZ.MethodsThe experimental settings were conducted based on our MDR-TB receiving BDQ-containing regimens. Sequential isolates obtained f...

The recommendation of the WHO Study Group to introduce MDT for the treatment of leprosy met with considerable resistance in Brazil. The National Department for Dermatological Disease (DNDS) advanced a number of arguments against the adoption of WHO MDT by Brazil (1) including: • significant risk of side-effects; • efficacy not proven; • lack of evidence to confirm: – speedier attainment of smea...

Erythema nodosum leprosum (ENL), or type II reaction, is a common complication of lepromatous leprosy that can cause significant patient debility. First-line therapy includes prednisone and thalidomide, with clofazimine reserved for patients who do not respond to first-line treatment. We present the case of a 33-year-old woman with ENL that failed to respond adequately to conventional therapy o...

We investigated the potential of a rapid colorimetric microassay based on the reduction of dimethylthiazol-diphenyltetrazolium bromide (MTT) for determining the growth of Mycobacterium avium-M. intracellulare complex (MAC) and MICs of clofazimine, resorcinomycin A, and the quinolone PD 127391 against MAC. The reduction of MTT was directly proportional to the number of viable bacteria. A compari...