ABSTRACT: This study evaluated the viability of Nellore cloned calves derived from somatic cell nuclear transfer (SCNT) and compare their with animals same breed in vitro fertilization (IVF). Thus, two groups were formed. Group I (GI) consisted 10 SCNT group II (GII) IVF. The differences detected between physical examination respiratory tract GI, which represented most common clinical-pathologi...

We report here the first successful use of embryonic nuclear transfer to create viable adult Drosophila melanogaster clones. Given the generation time, cost effectiveness, and relative ease of embryonic nuclear transplant in Drosophila, this method can provide an opportunity to further study the constraints on development imposed by transplanting determined or differentiated nuclei.

Diploid frog nuclei from differentiated somatic cells, transplanted into enucleated eggs to determine whether cell specialization generally involves irreversible genetic changes, have shown that nuclei from specialized somatic cells still contain the genes specifying the cell types and organ systems of swimming tadpoles. However, those tadpoles failed to feed and did not survive beyond the init...

The generation of infectious rabies virus (RV), a non-segmented negative-stranded RNA virus of the Rhabdoviridae family, entirely from cloned cDNA is described. Simultaneous intracellular expression of genetically marked full-length RV antigenome-like T7 RNA polymerase transcripts and RV N, P and L proteins from transfected plasmids resulted in formation of transcriptionally active nucleocapsid...

background : there are several methods, such as vaccination, to control visceral leishmaniasis. although there is no efficient vaccine, it seem dna vaccination with stimulates both cellular and humoral immunity apparently is the best way. the aim of this study was cloning and expression of lack gene, a 36kd protein, as a can-didate protein for vaccination against iranian l.infantum. methods: ir...

Background: Among wide range of bio-conservational strategies envisaged, recent accomplishments in the field of somatic cell nuclear transfer (SCNT) holds considerable promise due to its unique potential to decelerate or prevent rapid loss of animal genetic resources, and even to revive extinct species. This study was undertaken to investigate whether domestic sheep in vitro matured and enuclea...

To preserve Jeju black cattle (JBC; endangered native Korean cattle), a pair of cattle, namely a post-death cloned JBC bull and cow, were produced by somatic cell nuclear transfer (SCNT) in a previous study. In the present study, we examined the in vitro fertilization and reproductive potentials of these post-death cloned animals. Sperm motility, in vitro fertilization and developmental capacit...

The activation of antigen-specific B cell responses can be modulated by a number of T cell regulatory influences. It was first observed (1-3) that B cell responses to many antigens require the participation of T helper (Th) ~ cells. Studies (4, 5) using both heterogeneous and, more recently, cloned Th populations have shown that distinct B cell activation pathways exist in which Th cellB cell i...