We present a method to modify bacterial artificial chromosomes (BACs) resident in their host strain. The method is based on homologous recombination by ET-cloning. We have successfully modified BACs at two distinct loci by recombination with a PCR product containing homology arms of 50 nt. The procedure we describe here is rapid, was found to work with high efficiency and should be applicable t...

Spreadsheets are widely used in industry: it is estimated that end-user programmers outnumber programmers by a facto . However, spreadsheets are error-prone, numerous companies have lost money because of spreadsheet errors. One of the causes for spreadsheet problems is the prevalence of copypasting. This paper focuses on the methods of identifying data clone in spread sheets and their efficienc...

We constructed a cloning vector for use in the plant pathogenic bacterium Clavibacter michiganense subsp. michiganense. The vector pDM100 consists of a 3.2-kb restriction fragment of the Clavibacter plasmid pCM1 joined to a pBR325 derivative carrying the neomycin phosphotransferase of transposon Tn5 and the gentamicin acetyltransferase of Tn1696. Both antibiotic resistance genes are efficiently...

step. The presence of Vent DNA polymerase and PCR products other than the intended DNA linker, however, did not seem to interfere with subsequent reactions because ligations using both gel-purified and unpurified PCR products yielded a similar number of transformants. Eight transformants were tested, all of which contained the PCR product as an insert. The yield was considered to have high effi...

The recent advances in electronic and robotics industry have enabled the manufacturing of sensors capable of measuring a set of application-oriented parameters and transmit them back to the base station for analysis purposes. These sensors are widely used in many applications including the healthcare systems forming though a Wireless Body Sensor Networks. The medical data must be highly secured...

Precise manipulation of transgene expression in genetic model organisms has led to advances in understanding fundamental mechanisms of development, physiology, and genetic disease. Transgene construction is, however, a precondition of transgene expression, and often limits the rate of experimental progress. Here we report an expansion of the modular Gateway MultiSite recombination-cloning platf...

A sixty base pair DNA duplex containing the nucleotide sequence of the bacteriophage T5 early (T5P25) promoter has been constructed using a combination of chemical synthesis and enzymatic methods. Subsequent to cloning into pBR322, the promoter has been demonstrated to be biologically active being capable of directing the efficient expression of genes under its control. This serves as a prototy...

A quantum chaos cloning multi-objective evolutionary algorithm was proposed herein based on chaos search ergodicity, quantum computing efficiency and clonal selection theory of antibodies in artificial immune system. The qubits encoded initial population is used in the new algorithm, Chaos quantum rotation gates are introduced to update individuals, crowding distance is used to keep solution po...