The aim of the study was to fabricate and characterize composite macroporous hydrogels based on a hyaluronic acid/chitosan (Hyal/Ch) polyelectrolyte complex (PEC) loaded with homogeneously distributed hydroxyapatite nanoparticles (nHAp), evaluate them in vitro using mouse fibroblasts (L929), osteoblast-like cells (HOS) human mesenchymal stromal (hMSC). Hydrogel morphology as function nanopartic...

Many technological advancements of the past decade have contributed to improvements in the photon efficiency of the confocal laser scanning microscope (CLSM). The resolution of images from the new generation of CLSMs is approaching that achieved by the microscope itself because of continued development in digital imaging methods, laser technology and the availability of brighter and more photos...

K.G. Lieu and E.-H. Shim contributed equally to this paper. Correspondence to David A. Jans: [email protected] E.-H. Shim’s present address is Dept. of Urology, University of Alabama, Birmingham, AL 35233. Abbreviations used in this paper: CLSM, confocal laser scanning microscopy; FL, full length; IBB, importin- binding; IBBL, importin-–binding–like; IMP, importin; IP, immunoprecipitation...

Background Pseudomonas is one of the predominant microorganisms of chronic lung infections. Bacteria growing in biofilm often develop multicellular, three-dimensional structures known as micro colonies. Pseudomonas colonizes the lungs by forming biofilm micro colonies throughout the lung. In this study, using Pseudomonas spp. artificial biofilm was grown on flow cell chamber and images were tak...

Transmitted light and fluorescence microscopy often suffers from the limited Depth of Field (DOF) of microscope objectives. For this reason, plant cells can in most cases not completely be visualized in a single image at suitable magnification. In case of fluorescence signals, this problem can to some extent be circumvented by the use of confocal laser scanning microscopy (CLSM) which is able t...

function; (b) top row: MR-images acquired within 5min after contrast agent administration; middle row: MR-images 24h (column 1&2), 1.5h (column 3&4) and 48h (column 5&6) after injection; bottom row: CLSM of micellar and liposomal fluorescence (red) & autofluorescence (green) in border zone between remote and infarcted myocardium. a Contrast-enhanced cardiac MRI of vascular remodeling after myoc...

Dissociated DRG cell culture was performed and cytoskeletal vector was inserted into single neuronal cell by microinjection technique. Growth cone motility has been studied by timelapse imaging performed after 24 hrs with CLSM. By adding the stimulating factors growth cone circumference motility has increased after 60 minutes and this effect has drastically enhanced when incubated with VEGF + N...

The Dry Chaco in South America is a semi-arid ecoregion prone to dryland salinization. In this region, we investigated coarse-scale surface soil moisture (SM), temperature, salinity and vegetation, using L-band microwave brightness temperature (TB) observations retrievals from the Soil Moisture Ocean Salinity (SMOS) Active Passive (SMAP) satellite missions, Catchment La...