نتایج جستجو برای: colony forming unit assay
تعداد نتایج: 739725 فیلتر نتایج به سال:
Several lines of evidence indicate that macrophage inflamerythroid (BFU-E), but not by colony forming unit-erythroid matory protein-1a (MIP-1a) modulates the proliferation of (CFU-E), in a methylcellulose culture of purified human hematopoietic progenitor cells, depending on their maturaCD34 bone marrow cells. Although reverse-transcription tional stages. To clarify the mechanisms for the modul...
Many factors have been shown to influence bacterial transfer between surfaces, including surface type, bacterial species, moisture level, pressure, and friction, but the effect of inoculum size on bacterial transfer has not yet been established. Bacterial cross contamination rates during performance of common food service tasks were previously determined in our laboratory using nalidixic acid-r...
Indoor microbial exposure has been related to adverse pulmonary health effects. Exposure assessment is not standardized, and various factors may affect the measured exposure. The aim of this study was to investigate the seasonal variation of selected microbial exposures and their associations with temperature, relative humidity, and air exchange rates in Danish homes. Airborne inhalable dust wa...
A high frequency of transformation of bacteria of about 1—5xlO cells per /tg of supercoiled plasmid DNA (1, 2) is now routinely obtained when a dense suspension of Escherichia coli (MC1061/P3; Invitrogen) cells ( lxlO 0 cells/ml) are exposed to a brief (3 -5 msec) high voltage electrical pulse. However, if the DNA sample is dissolved in a ligation mixture with a high salt concentration, the tra...
Phenotype-driven strategies for screening Candida parapsilosis complex for molecular identification.
In this study, phenotypic methods presented >80% agreement with the molecular identification of 59 Candida parapsilosis complex. Growth at 15% NaCl or pH 7.0 significantly reduced cfu-counts of Candida orthopsilosis, suggesting these conditions may support the development of phenotypic methods for the differentiation of the cryptic species of C. parapsilosis complex.
In a study of occupational exposure to Bacillus thuringiensis, 20 exposed greenhouse workers were examined for Bacillus cereus-like bacteria in fecal samples and on biomonitoring filters. Bacteria with the following characteristics were isolated from eight individuals: intracellular crystalline inclusions characteristic of B. thuringiensis, genes for and production of B. cereus enterotoxins, an...
Objective(s): Ex vivo expansion of hematopoitic stem cells is an alternative way to increase umbilical cord blood (UCB)-CD34+ cells for bone marrow transplantation. For this purpose demineralized bone matrix (DBM) and mineralized bone allograft (MBA) as two scaffolds based on bone matrix and stem cell niche, were simultaneously used to enhance the effect of human mesenchymal pro...
Aflatoxins are a family of related bisfuranocoumarin compounds produced by fungi Aspergillus flavus and A. parasiticus. It has been reported that out of the known strains of A. flavus and A. parasiticus, only about one-half produce toxins. In present study the occurrence of total aflatoxin contamination in Indian maize samples collected from local market of Lucknow city were investigated by com...
An assessment of wood workers' exposure to airborne cultivable bacteria, fungi, inhalable endotoxins and inhalable organic dust was performed at 12 sawmills that process mainly coniferous wood species. In each plant, samples were collected at four or five different work sites (debarking, sawing, sorting, planing and sawing cockpit) and the efficiency of sampling devices (impinger or filter) for...
We compared urine culturing performed by the calibrated loop method with a screening system (BACTEC). A total of 852 urine specimens were examined by both the conventional loop method and the BACTEC system. With the loop method, 193 (22.6%) urine samples were positive, whereas with the BACTEC system, 185 (21.7%) were positive (sensitivity, 96.01%). At a breakpoint of 10(4) CFU/ml, eight false-n...
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