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A competitive ELISA for the determination of protein-A concentration in culture supernatants is described. The sensitivity of the assay is 50 ng/ml and it may be used to determine concentrations of protein-A up to 1000 ng/ml. The assay is specific, rapid and suitable for routine screening of protein-A producing microorganisms.

In the present study, we investigated if priming of autoreactive CD8(+) T cells would be inhibited by competitive peptides for major histocompatibility complex (MHC) class I binding. We used a mouse model of vitiligo which is induced by immunization of K(b)-binding tyrosinase-related protein 2 (TRP2)-180 peptide. Competitive peptides for K(b) binding inhibited IFN-γ production and proliferation...

of proteins at fluid interfaces, kinetics of adsorption of proteins at fluid interfaces, interfacial rheology, competitive adsorption of emulsifier-protein mixtures, hydrophile-lipophile balance.

The study of comorbidity can provide new insights into the pathogenesis disease and has important economic significance in clinical evaluation treatment difficulty, medical expenses, length stay, prognosis disease. In this paper, we propose a association prediction model DapBCH, which constructs cross-species biological network applies heterogeneous graph embedding to predict association. First...