This paper proposes the Intra Trustcenter Protocol (ITP), a flexible and secure management protocol for communication between arbitrary trustcenter components. Unlike other existing protocols (like PKCS#7, CMP or XKMS) ITP focuses on the communication within a trustcenter. It is powerful enough for transferring complex messages which are machine and human readable and easy to understand. In add...

A chemically defined medium was prepared which adequately supported growth of a vaccine strain of Pasteurella tularensis. This medium differed from those previously described in: (i) concentration of components, (ii) a requirement for calcium pantothenate to obtain increased growth, and (iii) a low initial pH. Varying the concentration of individual components up to 10 times the standard amount...

In this work, natural cashew apple juice was used as cultivation medium as an alternative to substitute brain heart infusion medium. The effect of aeration and juice supplementation with yeast extract on the production of hyaluronic acid in batch fermentation was also investigated. Similar levels of cell mass were obtained in inoculum using cashew apple juice supplemented with yeast extract or ...

Existing research on Smallto Medium-sized Manufacturers (SMMs) pays scant attention to factors affecting their growth in the contact of paradigms of competition and, particularly, segments of the supply chain in which they operate. This work adopts a supply chain perspective to propose a business definition and model for the process of the SMMs understanding of, responding to, andinf1uencing cu...

The reliability of the Mycobacteria Growth Indicator Tube (MGIT) 960 system for rapid detection of mycobacteria in clinical specimens was evaluated and compared to the radiometric method (BACTEC 460TB) and to mycobacterial culture on Lowenstein-Jensen (LJ) medium. Clinical specimens (n = 590) were tested without selection. A total of 121 (20.5%) isolates of mycobacteria were recovered; 98 (81.0...

Isolation of plasmid DNA from Escherichia coli is a daily activity in many molecular biology laboratories. A number of protocols and media recipes have been reported in an effort to make this process more efficient. Here we describe a growth medium that supports much higher E. coli cell densities and, concomitantly, a much higher yield of plasmid than previously reported for small-scale applica...

Random lasers are a developing class of light sources that utilize a highly disordered gain medium as opposed to a conventional optical cavity. Although traditional random lasers often have a relatively broad emission spectrum, a random laser that utilizes vibration transitions via Raman scattering allows for an extremely narrow bandwidth, on the order of 10 cm(-1). Here we demonstrate the firs...

Henry Darcy's experimental studies in 1856 of saturated water flowthrough a homogeneous porous medium contained in a vertical column have provided the basis for the quantitative description of fluid flow in a wide variety of both natural and engineered porous medium environmental systems. Extrapolation of Darcy's original observations and conclusions has led to several commonly applied equation...

The degradation of exogenous sphingomyelin was investigated in cultured fibroblasts from normal subjects and subjects with familial hypercholesterolemia, either in whole medium or in lipoprotein-deficient medium. When introduced in whole medium, sphingomyelin degradation was significantly decreased (about 1.5-fold) in heterozygotes, and dramatically (about 4-fold) in homozygotes from familial h...

Using two different liquid media and one conventional solid medium, a total of 57 mycobacterial isolates (Mycobacterium tuberculosis, n = 55; nontuberculous mycobacteria, n = 2) were recovered from 377 clinical specimens. The rates of recovery of M. tuberculosis were 96. 4% with the BACTEC MGIT 960 liquid medium, 92.7% with BACTEC 12B liquid medium, and 81.8% with the Löwenstein-Jensen (LJ) med...