PURPOSE To determine the effect of systemic anti-CD154 monoclonal antibody on the survival of orthotopic murine corneal transplants. METHODS BALB/c mice were used as recipients of syngeneic, multiple minor histocompatability (H)-disparate, or major histocompatibility complex MHC-mismatched corneal transplants. Recipient beds were either avascular (normal risk) or neovascularized (high risk). ...

AIM To describe a surgical technique for autologous limbal stem cell transplantation and the outcome of a series of patients with unilateral stem cell deficiency. METHODS A report of six consecutive patients who underwent autologous limbal stem cell transplantation is presented. The primary diagnosis included alkali burn (n=3), conjunctival intraepithelial neoplasia (CIN) (n=1), recurrent pte...

the past decade has witnessed the revival of amniotic membrane transplantation (amt) in ophthalmology. the importance of amt is due to its ability to reduce scarring and inflammation and to enhance wound healing and epithelialization, and also its anti-microbial properties. amniotic membrane has recently been used as a substrate for culturing limbal stem cells for transplantation. it has been u...

249 Eduard Zirm performed the first successful allogeneic corneal transplant in 1905,[1] making this corneal graft a pioneering event in early organ transplantation.[2] Despite this long history, our knowledge of corneal immune function and graft rejection pathophysiology and its prevention are lagging in comparison to solid organ transplantation. This is, to some extent, due to a misunderstand...

PURPOSE. High-risk corneal transplantation refers to grafting performed on inflamed and highly vascularized host beds. It represents a clinical dilemma because the rejection rate can be as high as 90%, irrespective of current treatment modalities. This study was conducted to investigate whether combined blockade of VEGFR-3 (vascular endothelial growth factor receptor-3) and VLA-1 (very late ant...

PURPOSE To determine the differential gene expression of chemokine species and leukocyte infiltration of grafts in the early pre-rejection postoperative period after high-risk (HR) compared to normal-risk (NR) corneal transplantation. METHODS Fully mismatched and syngeneic corneal grafts were performed in NR (avascular) and HR (vascularized) recipient beds of BALB/c murine hosts. Gene express...

Abstract Clinical case We report a of rheumatoid arthritis‐associated peripheral ulcerative keratitis (PUK) successfully treated with tocilizumab. Despite being systemic corticosteroids and several disease‐modifying antirheumatic drugs, evern though received corneal transplantation, the patient was still going to lose her sight. Tocilizumab resulted in good response control illness preventing p...

AIM To generate human embryonic stem cell derived corneal endothelial cells (hESC-CECs) for transplantation in patients with corneal endothelial dystrophies. MATERIALS AND METHODS Feeder-free hESC-CECs were generated by a directed differentiation protocol. hESC-CECs were characterized by morphology, expression of corneal endothelial markers, and microarray analysis of gene expression. RESUL...

RATIONALE Terrien's marginal corneal degeneration (TMD) is characterized by progressive peripheral corneal thinning. It appears primarily above the age of 40 years and is rare at younger ages. Spontaneous corneal perforation in TMD is a rare, but serious complication that may occur in childhood. PATIENT CONCERNS This review presents the case of a 16-year-old girl presented with blurred vision...

PURPOSE To report the adverse event of topical tosufloxacin administered after penetrating keratoplasty in one patient. CASE REPORT A 60-year-old female was referred to our hospital for treatment of vision loss due to corneal opacification, etiology was unknown. Slit lamp examination showed dense opacification in corneal stroma. Penetrating keratoplasty was performed on her left eye. She was ...