Introduction In the past decade several investigations have been reported on various hydrogels,1-3 particularly neutral polymer gels [e.g., poly(acrylamide) or poly(vinyl alco­ hol)] and weakly charged polyelectrolytes [e.g., poly­ (acrylic acid) or poly(styrenesulfonate)]. Less attention has been paid to neutralized polyelectrolytes in the presence of added salt. It is generally understood tha...

The helical tension of chromosomal DNA is one of the epigenetic landmarks most difficult to examine experimentally. The occurrence of DNA crosslinks mediated by psoralen photobinding (PB) stands as the only suitable probe for assessing this problem. PB is affected by chromatin structure when is done to saturation; but it is mainly determined by DNA helical tension when it is done to very low hi...

Purpose To analyze the errors associated to corneal power calculation using the keratometric approach in keratoconus eyes after accelerated corneal collagen crosslinking (CXL) surgery and to obtain a model for the estimation of an adjusted corneal refractive index (nkadj ) minimizing such errors. Methods Potential differences (ΔPc) among keratometric (Pk ) and Gaussian corneal power (PcGauss)...

München vorgelegt von Katrin Hahlen aus Köln 2004 Ehrenwörtliche Versicherung Diese Dissertation wurde selbständig und ohne unerlaubte Hilfsmittel angefertigt. "Testing kinesin's flexibility by crosslinking: Which domains need to be mobile to allow mobility?" "Testing kinesin's flexibility by crosslinking: Which domains need to be mobile to allow mobility?" "The role of the neck in fast kinesin...

The folding rates of two-state proteins have been found to correlate with simple measures of native-state topology. The most prominent among these measures is the relative contact order (CO), which is the average CO, or localness, of all contacts in the native protein structure, divided by the chain length. Here, we test whether such measures can be generalized to capture the effect of chain cr...

We demonstrate an enzyme stabilization approach whereby a model enzyme is PEGylated, followed by controlled chemical modification with glutaraldehyde. Using this stabilization strategy, size increases and aggregation due to intermolecular crosslinking are avoided. Immediately following synthesis, the PEGylated enzyme with and without glutaraldehyde modification possessed specific activities of ...

In recent years, scientists have expanded their focus from cataloging genes to characterizing the multiple states of their translated products. One anticipated result is a dynamic map of the protein association networks and activities that occur within the cellular environment. While in vitro-derived network maps can illustrate which of a multitude of possible protein-protein associations could...

An unprecedented three-level hierarchical entangled system was synthesized. The basic helical ribbons of the complex were entangled into 2D layers by self-penetration based on 1D → 2D polycatenation; a pair of layers interpenetrated, and crosslinked to construct the second self-penetrated bilayers which were further polycatenated into the final 3D entangled array.

Drug release from collagen matrices is in most cases governed by diffusion from swollen matrices but also enzymatic matrix degradation or hydrophobic drug/collagen interactions may contribute. To reduce water uptake and to prolong the release, insoluble collagen matrices have been chemically or dehydrothermally crosslinked. Assuming Fickian diffusion a one-dimensional model was developed and te...

1. Results of Collagen Crosslinking for Keratoconus ................................. 2 2. Patient Selection ................................................................................. 14 3. Patient Subjective Visual Function after Crosslinking ............................ 22 4. Corneal Shape and Topography Changes ............................................. 27 5. Higher Order Aberratio...