objectives: freezing and in vitro culture of testicular spermatozoa or tissue are reliable approaches for the management of azoospermic patients and could all for the possibility of multiple ivf-icsi procedures. however, the effect of cryopreservation duration on pregnancy outcome had been not fully examined. to analyze pregnancy outcome in patients underwent icsi procedure in relation to durat...

STUDY QUESTION Is there a better alternative to the conventional cryopreservation protocols for human testicular tissue banking? SUMMARY ANSWER Uncontrolled slow freezing (USF) using 1.5 M dimethylsulphoxide (DMSO) and 0.15 M sucrose as cryoprotectants appears to be a user-friendly and efficient method for the cryopreservation of human testicular tissue. WHAT IS KNOWN ALREADY Currently, tim...

Plant cryopreservation is useful for long term storage of clonal germplasm and endangered species. Clonally propagated crops which produce recalcitrant seeds cannot be easily conserved using conventional methods. Preservation plants in vitro limited to two years not ideal a very time. The need conserve plant genetic resources through techniques mitigate the effects climate change such as extinc...

The stromal compartment of adipose tissue harbors multipotent cells known as adipose-derived stem cells (ASCs). These cells can differentiate into various lineages including osteogenic, chrondrogenic, adipogenic, and neurogenic; this cellular fraction may be easily obtained in large quantities through a clinically safe liposuction procedure. Therefore, ASCs offer exceptional opportunities for t...

The present study was designed to test the hypothesis that addition of anticaspase cocktails (inhibiting caspases and thus blocking apoptosis) to the extenders increases the post-thaw viability of equine spermatozoa. The addition of caspase inhibitors failed to improve the acrosome and plasma membrane integrity of spermatozoa, suggesting that in equine sperm cryopreservation protocols, the addi...

BACKGROUND Previous studies have shown that trehalose, when used alone as a cryoprotective agent (CPA), can maintain the viability of living tissue after cryopreservation and therefore may be used clinically for the future banking of human fat grafts. OBJECTIVE The purpose of this study is to determine the optimal concentration of trehalose used as a CPA for the cryopreservation of adipose as...

PURPOSE The aim of this study was to investigate whether vitrification in the cryopreservation of periodontal ligament (PDL) cells could be useful for tooth banking. METHODS In step 1, primary cultured human PDL cells were cryopreserved in 100% conventional cryopreservation media and 100% vitrification media (ESF40 media) in different temperatures for 2 weeks. In step 2, a series of modified ...

In women, around 10% of cancers occur in those younger than 45 years old. Although aggressive chemotherapy/radiotherapy and bone marrow transplantation can cure more than 90% of girls and young women affected by disorders requiring such treatment, the ovaries are very sensitive to cytotoxic drugs, especially to alkylating agents. Therefore, these treatments can result in premature ovarian failu...

Background: Sperm cryopreservation has been associated with over production of reactive oxygen species (ROS). Buffalo spermatozoa are susceptible to ROS inducing damages due to insufficient level of cytoplsmic antioxidant along with high amount of poly unsaturated fatty acids composition in membrane structure. Therefore, appropriate antioxidant in buffalo cryopreservation extender would reduce ...

Light Source INTRODUCTION This study presents a new device prototype for visualization of physical effects associated with large-scale cryopreservation—the preservation of tissues at very low temperatures. Cryopreservation represents the only method for long-term preservation of biomaterials. While techniques for cryopreservation of single cells and small tissue structures are well established,...