The rising incidence of tuberculosis worldwide means an increasing burden on diagnostic facilities, so tests simpler than Ziehl-Neelsen staining are needed. Such tests should be objective, reproducible, and have at least as good a detection limit as 10(4) bacteria/ml. A capture enzyme-linked immunosorbent assay (ELISA) was developed for detection of lipoarabinomannan (LAM) in human sputum sampl...

The Trichoderma hamatum produces various secondary metabolites that can be used as Candida albicans. This research aimed to isolate T. from the coffee (Coffea canephora) rhizosphere and analyze fungal compounds control pathogenic fungus C. was isolated using dilution method, identification used, combining morphological molecular characteristics of ITS rDNA. potency albicans determined by antago...

Lymphocytes from uninfected, nonimmune mice can be stimulated to proliferate and produce antibody to diverse antigens in culture when exposed to the purified protein derivative of tuberculin or unheated tuberculin culture filtrate. Lymphocytes from numerous inbred strains of mice respond to tuberculin, but no true low responder strain has been found as yet. The evidence presented suggests that ...

Kedarcidin, a new chromoprotein antitumor antibiotic, was isolated from the culture broth of a novel actinomycete strain L585-6 (ATCC 53650). The antibiotic was recovered from the culture filtrate by adsorption to QAE ion exchanger and purified by successive application of gel filtration and ion exchange chromatography with Sephadex G-50 and DEAE-Sephadex, respectively. Kedarcidin is an acidic ...

A se}ectiye inactivating enzyme for the m-subunit of lactate dehydrogenase (LDH) was found in the cutture filtrate of PenieiM"m citrinum KE-1, newly isolated from soil. The enzyme was purified from the culture filtrate by ammonium sulfate fractionation, column chromatography on CM-Sepharose CL-6B, and gel filtration on Sephadex G-1OO. The purification was 124-fold with an activity yield of 81%....

This paper reports a solid-state fungal fermentation-based pre-treatment strategy to convert wheat straw into a fermentable hydrolysate. Aspergillus niger was firstly cultured on wheat straw for production of cellulolytic enzymes and then the wheat straw was hydrolyzed by the enzyme solution into a fermentable hydrolysate. The optimum moisture content and three wheat straw modification methods ...

In the course of our screening for novel antibacterial antibiotics produced by microorganisms, a strain termed KSM-2690was found to produce a new complex of triene/3-lactone antibiotics in the culture filtrate. The active principle was recovered from the broth filtrate with ethyl acetate extraction and could be divided into six components, three major components KSM-2690 A (1), B (2) and C (3),...

A new antifungal antibiotic, named ileumycin, was isolated from culture broth of streptomyces H 698-SY2, which was identified as S. lavendulae. The antibiotic was recovered from the culture filtrate by adsorption on Amberlite XAD-II and elution with aqueous methanol and was further purified by ion-exchange column chromatography on SE-cellulose and followed by partition chromatography on silica ...

After screeninig several lhundlred cultures of fungi, actinomvcetes, aid bacteria isolated fromii various soils for plant growtlh regulating activity, a fungus culture. F7-2578 (identified later as Peneicilliumiit pufrpurresceiis), was found( whIiclh had a (lwNarfing effect on pea planits. A foliage spray of the culture filtrate inhibite(d the gro)wvth of the stemls, producing shortened interno...