The optical density of a culture of lactococcus lactis MG1363 was reduced more than 60% during prolonged stationary phase. Reduction in optical density (autolysis) was almost absent in a culture of an isogenic mutant containing a deletion in the major autolysin gene, acmA. An acmA mutant carrying multiple coples of a plasmid encoding AcmA lysed to a greater extent than the wild-type strain did....

The initiation of many antigen-specific, T-dependent immune responses requires the participation of accessory cells that express Ia antigens (1, 2). Recent evidence indicates that the Ia antigen expression of macrophages, a principle class of accessory cells, is regulated by lymphokines: Ste inman et al. (3) have reported that culture supernatants of Trypanosoma cruzi-activated spleen cells enh...

The initiation of many antigen-specific, T-dependent immune responses requires the participation of accessory cells that express Ia antigens (1, 2). Recent evidence indicates that the Ia antigen expression of macrophages, a principle class of accessory cells, is regulated by lymphokines: Ste inman et al. (3) have reported that culture supernatants of Trypanosoma cruzi-activated spleen cells enh...

The aim of the present study was to elucidate whether the culture of cells recovered from induced sputum may represent a suitable model to evaluate cytokine and chemokine production by airway inflammatory cells. Sputum induction was performed in 21 normal subjects and 30 asthmatic patients. A total of 21 out of the 30 asthmatic patients were taking inhaled corticosteroids, while the remaining n...

Spherule and arthrospore cellular preparations were sonic-treated and separated into their respective supernatant and sediment components. Complement-fixation tests with antispherule and antiarthrospore pooled rabbit sera revealed that the soluble antigens exhibited more serological activity than the sediment preparations. After autoclaving, an arthrospore cellular antigen exhibited increased a...

BACKGROUND Factors that prevent colonic mucosal invasion by pathogenic Entamoeba histolytica are not understood. A key initial step in pathogenesis of injury induced by amoeba is adherence to target cells mediated by a surface glycoprotein lectin on E histolytica. Mucin degrading bacteria normally present in the colon lumen produce glycosidases that degrade soluble or cell surface glycoconjugat...

A novel platelet aggregation inhibitor, YM-254890, was isolated from the culture broth of strain QS3666. This strain was isolated from a soil sample collected at Okutama, Tokyo, Japan, and was identified as Chromobacterium sp. by morphological and physiological criteria. YM254890 was purified from the culture supernatant by solvent extraction, ODS and silica gel flash chromatography, followed b...

background and aims: influenza virus is a major pathogen involved in respiratory illnesses during winter seasons. a variety of diagnostic methods have been developed to identify influenza viruses in clinical specimen. methods: nasal and pharyngeal samples taken from patients were inoculated into madin-darby canine kidney (mock) cells and embryonated chicken eggs (eces). the culture media was as...

This study was designed to investigate the antifungal activity of Bacillus pumilus 344-3 against toxigenic fungi. In vitro co-incubation assay revealed that A. carbonarius AC82, niger AN8 and P. digitatum PD43 are most sensitive fungi bacterial compounds with zone inhibition 29.2 mm, 27.7 mm 27.1 respectively. The addition culture supernatant at low concentration in fungal growth medium stimula...

By using polyethylene glycol 1540, BW5147 AKR T lymphoma cells were fused with splenocytes from A/J mice treated so as to induce suppressor T cells specific for azobenzenearsonate (ABA). Of 576 microwells originally seeded, 132 demonstrated growing cell clones, 4 of which produced an ABA-binding supernatant factor. When tested in vivo for suppression of delayed-type hypersensitivity to ABA, two...