[1] Single-hole hydrogeologic experiments were conducted with a drillstring packer in Integrated Ocean Drilling Program Hole 1301B, which penetrates 318 m into 3.5-Ma upper oceanic basement on the eastern flank of the Juan de Fuca Ridge. Seven constant-rate injection tests were conducted in open hole, with the packer inflated at three depths in the deepest 166 m of the basement section. The pri...

Cytochrome c (cyt c) family proteins, such as horse cyt c, Pseudomonas aeruginosa cytochrome c551 (PA cyt c551), and Hydrogenobacter thermophilus cytochrome c552 (HT cyt c552), have been used as model proteins to study the relationship between the protein structure and folding process. We have shown in the past that horse cyt c forms oligomers by domain swapping its C-terminal helix, perturbing...

Voltammetry of cytochrome P450 (cyt P450) enzymes in ultrathin films with polyions was related for the first time to electronic and secondary structure. Heterogeneous electron transfer (hET) rate constants for reduction of the cyt P450s depended on heme iron spin state, with low spin cyt P450cam giving a value 40-fold larger than high spin human cyt P450 1A2, with mixed spin human P450 cyt 2E1 ...

The polymer solar cells were fabricated by a novel solution coating process, the roller painting. The roller painted film composed of poly(3-hexylthiophene) (P3HT) and [6,6]-phenyl-C61-butyric acid methyl ester (PCBM) has smoother surface than the spin coated film. Since the roller painting is accompanied with shear and normal stresses and is also a s low drying process, the process induces eff...

OBJECTIVE To determine the number of fatalities related to roller coasters and examine factors common to multiple incidents. METHODS A case was defined as the death of a person, which was associated with a roller coaster in the United States between 15 May 1994 and 14 May 2004. Cases were identified from four DATA SOURCES (1) Consumer Product Safety Commission, (2) Lexis-Nexis, (3) Medline,...

The structural transformation of cytochrome c (cyt c) and its heme-free precursor, apo cyt c, induced by negatively charged sulfonated polystyrene (SPS) with different charge density (degree of sulfonation) and chain length was studied to understand the factors that influence the folding and unfolding of the protein. SPS forms stable transparent nanoparticles in aqueous solution. The hydrophobi...

Fluorescence resonance energy transfer-sensitized emission of the yellow cameleon 3.60 was used to study the dynamics of cytoplasmic calcium ([Ca(2+)](cyt)) in different zones of living Arabidopsis (Arabidopsis thaliana) roots. Transient elevations of [Ca(2+)](cyt) were observed in response to glutamic acid (Glu), ATP, and aluminum (Al(3+)). Each chemical induced a [Ca(2+)](cyt) signature that ...

During in vitro fertilization of wheat (Triticum aestivum, L.) in egg cells isolated at various developmental stages, changes in cytosolic free calcium ([Ca2+]cyt) were observed. The dynamics of [Ca2+]cyt elevation varied, reflecting the difference in the developmental stage of the eggs used. [Ca2+]cyt oscillation was exclusively observed in fertile, mature egg cells fused with the sperm cell. ...