Cell-free extracts of ikfucor rouxii contain enzymes which synthesize uridine diphosphate &lcetyl-D-glucosamine from IV-acetyl-n-glucosamine. Procedures are described to prepare radiolabeled UDP-GlcNAc using cell-free extracts or living cells of this fungus. This sugar nucleotide serves as the precursor for chitin synthesis. Free N-acetyl-n-glucosamine is a strong activator of chitin synthesis....

An efficient carcinogenic metal biosorbent was prepared by immobilizing the microalgae Auxenochlorella protothecoides (AP) within a sugarcane bagasse (SB) lignocellulosic matrix. The characterization of AP-SB as proposed performed various spectroscopic methods, including Fourier Transform Infrared (FTIR), Scanning Electron Microscopy (SEM), Energy-Dispersive X-ray Spectroscopy (EDX), and Thermo...

Transport of D-glucose was studied in Percoll-gradient-purified rat liver lysosomes. D-Glucose uptake had a Km of 22 mM and a t1/2 of approx. 30 s. D-Fucose, 2-deoxyglucose and methyl alpha-glucoside were the most effective competitors for uptake of D-glucose, although D-galactose, D-mannose, D-xylose and L-fucose also appeared to compete for uptake. L-Glucose was a poor competitor for uptake. ...

d-[1-(14)C]Galactosamine appears to be utilized mainly by the pathway of galactose metabolism in rat liver, as evidenced by the products isolated from the acid-soluble fraction of perfused rat liver. These products were eluted in the following order from a Dowex 1 (formate form) column and were characterized as galactosamine 1-phosphate, sialic acid, UDP-glucosamine, UDP-galactosamine, N-acetyl...