The inhibitory allelopathic activity of Pueraria montana (Kudzu), and activities two putative allelochemical isoflavones, puerarin daidzein, were evaluated using the protoplast co-culture method with digital image analysis lettuce as a recipient (DIA-PP method). Cotyledon protoplasts Kudzu isolated Cellulase R10 Driselase 20 in 0.6 M mannitol solution. Optimal hormonal condition density for gro...

Inflammatory bowel disease (IBD) comprises two major subtypes, ulcerative colitis (UC) and Crohn’s disease, which are multifactorial diseases that may develop due to genetic susceptibility, dysbiosis, or environmental factors. Environmental triggers of IBD include food-borne factors, a previous nationwide survey in Japan identified pre-illness consumption isoflavones as risk factor for UC. Howe...

The status of glycoconjugates (protein bound hexose, hexosamine, sialic acid and fucose) in plasma or serum serve as potential biomarkers for assessing tumor progression and therapeutic interventions. Aim of the present study was to investigate the protective effect of two major soy isoflavones, genistein and daidzein, in combination on the status of glycoconjugates in plasma, erythrocyte membr...

The aims of this study were to investigate the impact of soybean isoflavones (SIFs) on the cellular oxidative stress levels in hormone-sensitive type (LNCap) and hormone-insensitive type (DU145) of prostate cancer, and to elucidate the main components and their roles in the transformation of androgen dependency (AD)/ androgen independency (AI). The LNCap and DU145 cells were treated with differ...

Flavonoids are biologically active polyphenolic compounds widely distributed in plants. Recent research has focused on high dietary intake of flavonoids because of their potential to reduce the risks of diseases such as cardiovascular diseases, diabetes, and cancers. We report here the effects of plant flavonoids on catecholamine signaling in cultured bovine adrenal medullary cells used as a mo...

Valid food-frequency questionnaires (FFQ) need to be developed to assess isoflavone intake in investigations of its possible association with the lower incidence of breast and prostate cancer in Asian countries. We investigated the validity and reproducibility of isoflavone (daidzein and genistein) intakes from self-administered semiquantitative FFQ used in the JPHC Study (Japan Public Health C...

Puerarin (daidzein-8-C-glucoside) is the major bioactive isoflavone of kudzu root (the root of Pueraria lobata). Its metabolic fate, however, is not well-known. In this study, a sensitive and specific LC-ESI-MS/MS method for the determination of puerarin and its metabolites daidzein, dihydrodaidzein, and equol was developed for their analysis in biological samples. Two new metabolites of puerar...

The zoospores of Phytophthora sojae are chemotactically attracted to the isoflavones genistein and daidzein that are released by soybean roots. In this study we have examined the response of P. sojae zoospores to a wide range of compounds having some structural similarity to genistein and daidzein, including isoflavones, flavones, chalcones, stilbenes, benzoins, benzoates, benzophenones, acetop...

Experimental Biology and Medicine Charlotte Atkinson, Cara L. Frankenfeld and Johanna W. Lampe to Human Health Gut Bacterial Metabolism of the Soy Isoflavone Daidzein: Exploring the Relevance 2005, 230:155-170. Experimental Biology and Medicine http://ebm.rsmjournals.com/content/230/3/155 Updated information and services can be found at: http://ebm.rsmjournals.com/content/230/3/155#BIBL This ar...