A nucleoside kinase has been purified about 1680-fold from L1210 leukemia cells with deoxyadenosine as the phosphate acceptor and with adenosine triphosphate as phosphate donor. The molecular weight of the enzyme, determined by Sephadex G-200 column chromatography, was found to be about 100,000. Purified enzyme, after the final step of purification and also after disc gel electrophoresis (Rf 0....

Occupational nitrosamine exposures from a rubber vehicle seal (VS) curing operation were compared with the peripheral blood lymphocyte concentrations of two nitrosamine-related DNA adducts, N(7)-methylguanine (N(7)mdG) and O(6)-methylguanine (O(6)mdG), and with the activity of the enzyme that repairs O(6)mdG adducts, O(6)-alkylguanine-DNA alkyltransferase (AGT). The occupational personal breath...

BACKGROUND Adverse biological effects from airborne pollutants are a primary environmental concern in highly industrialized areas. Recent studies linked air pollution exposures with altered blood Deoxyribo-nucleic acid (DNA) methylation, but effects from industrial sources and underlying biological mechanisms are still largely unexplored. METHODS The Ma Ta Phut industrial estate (MIE) in Rayo...

The mutT protein, which prevents A:T -P CiG transversions during DNA replication, has the following enzymatic properties. Although it prefers dGTP as a substrate, it hydrolyzes all of the canonical nucleoside triphosphates to some extent. It has no activity in the absence of divalent cations, is maximally activated by magnesium ions, and has a pH optimum of 9.0. Nucleoside triphosphates are hyd...

A product of oxidative metabolism, 8-oxodeoxyguanosine triphosphate (8-O-dGTP), readily pairs with adenine during DNA replication, ultimately causing A.T-->C.G transversions. This study utilized 8-O-dGTP as a probe to examine the fidelity of the leading and lagging strand replication apparatus in extracts of HeLa cells. Simian virus (SV) 40 T antigen-dependent DNA replication reactions were per...

BACKGROUND Multiplexed detection of low-level mutations presents a technical challenge for many technologies, including cancer gene panels used for targeted-resequencing. Analysis of mutations below approximately 2%-5% abundance in tumors with heterogeneity, samples with stromal contamination, or biofluids is problematic owing to increased noise from sequencing errors. Technologies that reduce ...

Adenosine but not cytidine at 2 HIMdelayed metaphase some what. Nucleolar reconstruction was retarded in cells after treatment with adenosine, deoxyadenosine, and certain combinations of agents including deoxyribonueleosides. A nucleolar change resembling that induced by adenosine or 6-thioguanine was produced in interphasic cells by a combina tion of deoxyadenosine, deoxyguanosine, and deoxycy...