We are reporting an efficient method for magnetic microparticle based DNA extraction on a segmented flow microfluidic platform [1]. The droplet splitter and the magnetic setup were optimized separately to achieve highest separation efficiency. The novel asymmetric droplet splitter was developed allowing for dynamic control and arbitrary selection of droplet splitting ratios between 50:50 and 95...

Thermal fluctuations significantly affect the behavior of microscale systems rotating in shear flow, such as microvortexes, microbubbles, rotating micromotors, microactuators and other elements of lab-on-a-chip devices. The influence of Brownian torque on the motion of individual magnetic microparticles in a rotating magnetic field is experimentally determined using optical tweezers. Rotational...

Summary of advances in synthesis and application optical ratiometric nano- microparticle sensors for vitro vivo detection pH, oxygen, ROS, ions, biomarkers cell microenvironment.

Diagnostic methods are developed to measure the microparticle charge Q and two plasma parameters, electron temperature Te, and ion density ni, in the main plasma region of a dusty plasma. Using video microscopy to track microparticles yields a resonance frequency, which along with a charging model allows an estimation of Q and Te. Only measurements of microparticle position and velocity are req...

Possible mechanisms of particle attraction providing formation the field aligned microparticle strings in complex plasmas at elevated gas pressures are theoretically investigated light Plasmakristall-4 (PK-4) experiment on board International Space Station. The interaction energy is addressed by two different approaches: (i) using dynamically screened wake potential for small Mach numbers deriv...

Cerebral malaria (CM) is a severe complication of Plasmodium falciparum infection responsible for thousands of deaths in children in sub-Saharan Africa. CM pathogenesis remains incompletely understood but a number of effectors have been proposed, including plasma microparticles (MP). MP numbers are increased in CM patients' circulation and, in the mouse model, they can be localised within infla...

Endothelial microparticles are effectors of endothelial damage; however mechanisms involved are unclear. We examined the effects of eMPs on cultured endothelial cells (ECs) and isolated vessels and investigated the role of eMP-derived reactive oxygen species (ROS) and redox signaling in these processes. eMPs were isolated from EC media and their ability to directly produce ROS was assessed by l...

Background: We aim to investigate whether circulating microparticle (CMPs) subsets were raised amongst people presenting with a new diagnosis of HIV and advanced immune suppression in Malawi, they associated arterial stiffness. Methods: Microparticle characterisation carotid femoral Pulse Wave Velocity (cfPWV) carried out cohort ad...