1. A study has been made of the non-specific fluorescent staining of splenic imprints treated with fluorescent sheep antibody globulins. 2. In tissue imprints made with the spleens of antigen-stimulated animals, no morphological distinction was evident between areas showing non-specific fluorescence and specific fluorescence. 3. Elimination of non-specific fluorescence was not achieved by any o...

Guinea pig liver transglutaminase has been purified 230fold in high yield by means of diethylaminoethyl cellulose chromatography of liver homogenate supernatant fluid, precipitation of the enzyme with protamine, selective extraction with ammonium sulfate solution, and rechromatography on the cellulose absorbent. Estimates of molecular weight made by several procedures, 76,900 f 5,000 (sedimenta...

Crude and purified staphylococcal alpha-toxin were used to demonstrate that the platelet-damaging effect of crude alpha-toxin represents a fifth activity of the alpha-toxin molecule. The homogeneity of the purified toxin employed was demonstrated by ultracentrifugation, Ouchterlony, and immunoelectrophoretic methods. Continuous-flow electrophoretic migration studies demonstrated under a variety...

Experiments by Bendich et al. (1, 2) and by Polli and Shooter (3, 4) have suggested that the deoxyribonucleic acids might be tissue specific. Bendich et al. (1) found that the DNA of rat spleen, kidney, brain, and intestine could be distinguished by their chromatographic profiles on diethylaminoethyl-cellulose. The chromatographic differences between rat kidney and brain DNA were particularly p...

A purification procedure is described for two major toxic proteins from seeds of Abrus precatorius by successive chromatography on diethylaminoethyl-Sephadex A-50, carboxymethyl-cellulose, and DEAE-cellulose. The two toxins obtained both appeared homogeneous as judged by electrophoresis and analytical ultracentrifugation. Abrin A, the more positively charged protein obtained from the DEAE-cellu...

Although there have been a number of studies on the physical properties of bovine prothrombin (2-5) and, to some extent, equine prothrombin (6), there are yet few data available on preparations obtained from human plasma. This is largely due to the fact that methods customarily employed to isolate prothrombin from bovine plasma, when applied to human plasma, inevitably result in products of inf...

Background Nicotine, cotinine and cadmium are harmful components of cigarettes that have an effect on human reproductive function. Although the effects of cigarette smoke on male reproductive function is characterized in several articles its mechanism of action is still unknown. In the present study, we investigate the effect of nicotine, cotinine and cadmium on human sperm creatine kinase acti...

A new method for the chromatographic fractionation of soluble ribonucleic acid (sRNA) is described. A gradient of increasing salt and pH is employed to elute sRNA from diethylaminoethyl Sephadex. The resolution of sRNA species for 10 different amino acids obtained with this method is presented. There is evidence that resolution is dependent upon a change in sRNA from a partially denatured state...