A monolithic enzymatic microreactor was prepared in a fused-silica capillary by in situ polymerization of acrylamide, glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) in the presence of a binary porogenic mixture of dodecanol and cyclohexanol, followed by ammonia solution treatment, glutaraldehyde activation and trypsin modification. The choice of acrylamide as co-monomer was foun...

To compare digestion in the forestomach of llamas and sheep, the animals were fed four different diets: hay alone (H), low in nitrogen; the same hay with soybean meal (HS), with barley (HB) and with both soybean meal and barley (HSB). The sheep intakes were restricted to obtain about the same intake level in the two species. On average, the digestibilities of DM, OM and NDF were significantly h...

UNLABELLED Effective digestive enzymes are crucial for successful islet isolation. Supplemental proteases are essential as they synergize with collagenase for effective pancreas digestion. The presence of tryptic-like activity has been implicated in efficient enzyme blends and the present study aimed to evaluate if addition of clostripain, an enzyme with tryptic-like activity, could improve eff...

Eleven 1-pool, seven 2-pool, and three 3-pool models were compared in fitting gas production data and predicting in vivo NDF digestibility and effective first-order digestion rate of potentially digestible NDF (pdNDF). Isolated NDF from 15 grass silages harvested at different stages of maturity was incubated in triplicate in rumen fluid-buffer solution for 72 h to estimate the digestion kinetic...

A study was initiated to construct a micro-reactor for protein digestion based on trypsin-coated fused-silica capillaries. Initially, surface plasmon resonance was used both for optimization of the surface chemistry applied in the preparation and for monitoring the amount of enzyme that was immobilized. The highest amount of trypsin was immobilized on dextran-coated SPR surfaces which allowed t...

The effectiveness of microwave-assisted protein digestion in different well positions of a 96-well microplate was investigated where microwave-assisted protein digestion of bovine serum albumin was performed in 10 different wells of a 96-well microplate in a microwave oven. Similarly increased sequence coverages (~70%) were generally observed for the 10 microwaveassisted protein digestion sampl...

livestock are an integral part of the agriculture sector and encompass a great impact on the national economy. an eco-friendly alternative to enhance production, prevent and treat disease conditions of animal is a great challenge for animal nutritionists. keeping farm animals healthy is necessary to obtain healthy animal products. the use of naturally occurring compounds like herbs, herbal prep...

reaction constants and heat release during thermophilic aerobic digestion of typical secondary and mixed sludges were determined. maximum reaction constant, determined by measuring the amounts of biodegradable volatile suspended solids (bvss) removed, occurred in the temperature range of 55-60 ºc. measurement of heat releases were carried out, at the same range, by the aid of a temperature prog...

anaerobic digestion is the breakdown of biodegradable organic material by microorganisms in the absence of oxygen or in an oxygen-starved environment.this technology is superior to the landfilling and also the aerobic composting. the aim of the present study was to examine whether the effluent volatile fatty acids from the anaerobic acidogenesis of the food waste can be used du to its high valu...